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A6410 Sigma

Apyrase from potatoes

ATPase ≥200 units/mg protein, lyophilized powder

Synonym: Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

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Properties

Related Categories 3.6.x.x Acting on acid anhydrides, 3.x.x.x Hydrolases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
type   Grade VI
form   lyophilized powder
secondary activity   ≤15 % of base activity ADPase
composition   Protein, 30-60%
solubility   H2O: soluble 1.0 mg/mL
foreign activity   Acid Phosphatase ≤2% of base activity
storage temp.   −20°C

Description

Application

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .

Other Notes

This grade has an exceptionally high ATPase/ADPase ratio.

Packaging

100, 200, 500 units in glass bottle

Sold on the basis of ATPase units.

Preparation Note

Derived from white potato

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Physical form

Partially purified, lyophilized powder containing potassium succinate buffer salts.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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