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  • A6531 - Anti-Mouse IgG (whole molecule)−Agarose antibody produced in goat

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A6531 Sigma

Anti-Mouse IgG (whole molecule)−Agarose antibody produced in goat

affinity isolated antibody, suspension

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Properties

Related Categories Affinity Chromatography, Agarose, Alphabetical Index, Antibodies, Antibody Purification and Characterization,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   suspension
species reactivity   mouse
extent of labeling   ≥5 mg per mL
application(s)   Ouchterlony double diffusion: suitable
  immunoprecipitation: suitable
matrix   Cross-linked 4% beaded agarose
matrix activation   cyanogen bromide
matrix spacer   1 atom
capacity   0.4 mg/mL, resin binding capacity (mouse IgG)
conjugate   agarose conjugate
storage temp.   2-8°C

Description

Immunogen

IgG isolated from pooled normal mouse serum

Packaging

2, 5, 25 mL in glass bottle

Physical form

Suspension in 0.5 M NaCl containing 0.01% thimerosal.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunoprecipitation (1 paper)

Goat Anti-Mouse IgG (whole molecule)-Agarose antibodies have been used for immunoprecipiation assays. The product can also be used for Ouchterlony double diffusion applications.

Soluble antigens from meningococci extracts were mixed with ascitic fluid containing primary antibody and 50 μl of agarose-linked goat anti-mouse IgG for 5 hours at 4 °C.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Goat Anti-Mouse IgG (whole molecule)-Agarose antibodies bind to all mouse IgG subclasses.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
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Peer-Reviewed Papers
15

References

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