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A6531 Sigma

Anti-Mouse IgG (whole molecule)−Agarose antibody produced in goat

affinity isolated antibody, suspension

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Properties

Related Categories Affinity Chromatography, Agarose, Alphabetical Index, Antibodies, Antibody Purification and Characterization,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   suspension
species reactivity   mouse
extent of labeling   ≥5 mg per mL
application(s)   Ouchterlony double diffusion: suitable
  immunoprecipitation: suitable
matrix   Cross-linked 4% beaded agarose
matrix activation   cyanogen bromide
matrix spacer   1 atom
capacity   0.4 mg/mL, resin binding capacity (mouse IgG)
conjugate   agarose conjugate
storage temp.   2-8°C

Description

Immunogen

IgG isolated from pooled normal mouse serum

Packaging

2, 5 mL in glass bottle

Physical form

Suspension in 0.5 M NaCl containing 0.01% thimerosal.

Application

Goat Anti-Mouse IgG (whole molecule)-Agarose antibodies have been used for immunoprecipiation assays. The product can also be used for Ouchterlony double diffusion applications.

Soluble antigens from meningococci extracts were mixed with ascitic fluid containing primary antibody and 50 μl of agarose-linked goat anti-mouse IgG for 5 hours at 4 °C.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Goat Anti-Mouse IgG (whole molecule)-Agarose antibodies bind to all mouse IgG subclasses.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

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