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A6535 Sigma

Apyrase from potatoes

ATPase ≥200 units/mg protein, lyophilized powder

Synonym: Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

Properties

Related Categories 3.6.x.x Acting on acid anhydrides, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
Quality Level   PREMIUM
type   Grade VII
form   lyophilized powder
secondary activity   ≥50 % of base activity ADPase
composition   Protein, 25-60%
solubility   H2O: soluble1.0 mg/mL
Market Segment   Diagnostic Assay Manufacturing
foreign activity   Acid Phosphatase ≤2% of base activity
storage temp.   −20°C

Description

Application

At least two isoenzymes are found in different varieties of S. tuberosum:4,5 one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation 2.

Other Notes

This grade has a low ATPase/ADPase ratio.

Packaging

Sold on the basis of ATPase units.

Preparation Note

Derived from red potato

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Physical form

Partially purified, lyophilized powder containing potassium succinate buffer salts.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis 3. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

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