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A6559 Sigma

Adenosine 5′-triphosphate disodium salt solution

Crystalline ATP, HPLC purified, aqueous solution for RNA transcription

Synonym: ATP

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Properties

Related Categories Biochemicals and Reagents, Molecular Biology, Molecular Biology Reagents, Nucleosides, Nucleotides, Oligonucleotides, Nucleotides,
InChI Key   TTWYZDPBDWHJOR-IDIVVRGQSA-L
form   liquid
concentration   100 mM in H2O (adjusted to pH 7 with Trizma®
base)
foreign activity   DNase, RNase, none detected
shipped in   dry ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

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Trizma is a registered trademark of Sigma-Aldrich Co. LLC

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Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Frequently Asked Questions

How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/General_Information/shippingandlong-termstoragev3.Par.0001.File.tmp/shippingandlong-termstoragev3.pdf
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Can Product A6559, Adenosine 5′-triphosphate disodium salt solution, be used in molecular biology applications?
This is a Molecular Biology grade ATP. It is certified free of DNase and RNase. This product is recommended for any typical molecular biology application.
Can this preparation of Product A6559, Adenosine 5′-triphosphate disodium salt solution, be used as an ATP standard?
This preparation of ATP is not recommended for use as an ATP standard. The recommended ATP products for use as a standard in bioluminescence experiments is Product Code FL-AAS (preweighed vials of 1 mg each = 2 micromole).
What is meant by 25UMO in the size description of Product A6559, Adenosine 5′-triphosphate disodium salt solution?  What is the product dissolved in?
25UMO means 25 micromoles.  The 25 UMO size is equivalent to 250 μl of a 100 mM solution. This product is a 100 mM solution in water; the solution contains no buffer or salts.
Can Product A6559, Adenosine 5′-triphosphate disodium salt solution, be used for in vitro transcription?
The purity of this product should be sufficient for use in T7 RNA polymerase-catalyzed in vitro transcription assay, but we do not test the product for use in that application in our quality control process.
How long can aqueous ATP solutions like Product No. A6559 be stored?
Aqueous solutions of ATP are stable for months when frozen in solution at -15 ºC and for approximately one week at 0 ºC.  As with most products, we guarantee the quality of this material for 1 year when stored unopened at the recommended storage temperature (-20°C in this case).
What is the binding affinity of ATP (Product No. A6559) for Mg++ and other metal ions?
ATP has intrinsic metal binding affinity. The binding constant for various metals are (given as per mole): Mg++, 9554; Na+, 13; Ca++, 3722; K+, 8; Sr++, 1381; Li+, 25.  The ability of ATP to chelate magnesium results in effective Mg++ ion concentrations lower than the total Mg ion concentration.  For example, in a reaction with 1.5mM Mg++ and 0.2 mM ATP, the effective Mg ion concentration is ~1.3mM.
What is the millimolar extinction coefficient for ATP, Product No. A6559?
The millimolar extinction coefficient (EmM) is 15.4, measured at 259 nm for a sample in 100 mM phosphate buffer, pH 7.0.
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