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  • A6561 - Monoclonal Anti-Biotin–Alkaline Phosphatase antibody produced in mouse

A6561 Sigma

Monoclonal Anti-Biotin–Alkaline Phosphatase antibody produced in mouse

clone BN-34, purified immunoglobulin, buffered aqueous glycerol solution

Synonym: Monoclonal Anti-Biotin



Related Categories Alphabetical Index, Antibodies, Antibodies to Biotin, B-BI, Biotin and Biotin Antibodies,
biological source   mouse
antibody form   purified immunoglobulin
clone   BN-34, monoclonal
form   buffered aqueous glycerol solution
application(s)   dot blot: 1:10,000 using human IgG and biotin-monoclonal anti-human IgG
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil and biotin-monoclonal anti-human IgG
  indirect ELISA: 1:30,000 using human IgG and biotin-monoclonal anti-human IgG
  western blot (chemiluminescent): 1:10,000 using human IgG and biotin-monoclonal anti-human IgG
isotype   IgG1
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C



Biotin-KLH conjugate

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide.


Detection of biotinylated glycoproteins in fungal spores were detected by western blot using alkaline phosphatase-conjugated monoclonal anti-biotin antibody at 1:5000 in PBS.

Monoclonal Anti-Biotin-Alkaline Phosphatase antibody has been used for ELISA applications.

The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

General description

Biotin is a carboxylase co-enzyme that regulates the metabolism of amino acids, glucose and fatty acids. This co-enzyme has been implicated in processes such as immune responses, cell division and fetal growth. Biotin may also be involved in the epigenetic modifications of chromatin.

The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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