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  • A6715 - Anti-Mouse IgG1 (heavy chain specific)−Agarose antibody produced in goat

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A6715 Sigma

Anti-Mouse IgG1 (heavy chain specific)−Agarose antibody produced in goat

affinity isolated antibody, saline suspension

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Properties

Related Categories Agarose, Alphabetical Index, Antibodies, Antibody Purification and Characterization, Antibody-Agaroses,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   saline suspension
application(s)   immunoelectrophoresis: suitable
capacity   0.4 mg binding capacity
conjugate   agarose conjugate
storage temp.   2-8°C

Description

Immunogen

Purified mouse IgG

Packaging

2, 5 mL in glass bottle

Physical form

Suspension in 0.5 M NaCl and 0.01% thimerosal.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse IgG1 (heavy chain specific)-Agarose antibody is suitable for immunoelectrophoresis. It was used for immunoprecipitation to remove excess IgG1 using using recombinant mouse lengsin and hamster vimentin.

Western blot for detection of IgM heavy chains was performed using goat anti-mouse IgG heavy chain specific.

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG1 is the most abundant of the IgG subclass
The antibody is purified by immunospecific purification and is covalently attached to cyanogen bromide activated cross-linked beaded agarose. Anti-Mouse IgG1 (heavy chain specific)−Agarose antibody is specific for mouse IgG1 when tested against purified mouse IgA, IgG (all subclasses) and IgM.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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