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A6930 Sigma

β-N-Acetylhexosaminidase

recombinant, expressed in E. coli, buffered aqueous solution

Synonym: β-N-Acetyl-D-hexosaminide N-acetyl-hexosaminohydrolase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Biochemicals and Reagents, Carbohydrate-active Enzymes, Enzyme Class Index,
recombinant   expressed in E. coli
form   buffered aqueous solution
foreign activity   α-Glucosidase <1%
  α-mannosidase, β-hexosaminidase, α-fucosidase, neuraminidase, and proteases, none detected (enzyme is expressed in glycosidase-free host)
  β-Galactosidase <2%
storage temp.   2-8°C
Gene Information   human ... HEXA(3073)

Description

Biochem/physiol Actions

Active on N-acetyl-β-D-glucosaminides and N-acetyl-β-D-galactosaminides.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at pH 5.0 at 37°C.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl, 10 mM DTT

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

WGK Germany 
2

Documents

Certificate of Analysis

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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inhibitor

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A0500 Acetamide, ~99% (GC)

substrate

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M2133 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide, β-N-acetylhexosaminidase substrate
N9376 4-Nitrophenyl N-acetyl-β-D-glucosaminide, ≥99% (TLC)
N4006 Naphthol AS-BI N-acetyl-β-D-glucosaminide, β-hexosaminidase substrate

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