Accutase® solution sterile-filtered, suitable for cell culture

Properties

Related Categories	Cell Culture, Cell Dissociation, Enzymes, Reagents and Supplements
sterility	sterile-filtered
pH	6.8-7.8
suitability	suitable for cell culture
storage temp.	−20°C

Description

Frequently Asked Questions

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Application

Proven effective in detaching primary fibroblasts, endothelial cells, neurons, tumor cell lines, and insect cells. Performs exceptionally well in detaching cells for analysis of cell surface markers, virus growth assay, and flow cytometry as well as bioreactor scale-up. Does not contain mammalian or bacterial-derived products.

Features and Benefits

Ready-to-use liquid consisting of a proprietary mixture of proteolytic and collagenolytic enzymes for use in dissociation of cells from standard and adhesion-coated plasticware.

Formula variant

Prepared in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH₂PO₄, 8 g/L NaCl, and 1.15 g/L Na₂HPO₄) containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red. Refer to Product Data Sheet on the website for product usage information.

Other Notes

Special formulation that gently and rapidly dissociates tissues for cell isolation and propagation. This combines protease and collagenolytic activities which maximizes its versatility for cell detachment of adherent cells and tissue dissociation.