• USA Home
  • A7054 - ACCUSPIN System-Histopaque®-1077

EMAIL THIS PAGE TO A FRIEND
A7054 Sigma

ACCUSPIN System-Histopaque®-1077

Purchase

Properties

Related Categories ACCUSPIN and Histopaque, Accuspin, Cell Biology, Density Gradient Media, General Stem Cell Biology,
sterility   aseptically filled
shelf life   Expiry date on the label.
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

ACCUSPIN System-HISTOPAQUE-1077 employs centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit"). The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.

General description

γ-irradiated 50 mL polypropylene tubes fitted with a HDPE porous barrier and aseptically filled with 15 mL of Histopaque 1077. Each tube will separate 15-30 mL of anticoagulated blood.

Quantity

Package contains 12 prefilled tubes

Legal Information

Accuspin is a trademark of Sigma-Aldrich Co. LLC

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

Protocols & Applications

Video Procedure for Accuspin

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What are the most commonly used anticoagulants for the Accuspin tubes (Product A7054)?
EDTA or heparin are the most commonly used anticoagulants.  Heparin however should not be used when performing RNA assays.  EDTA can interfere with certain calcium binding assays.  Other anticoagulants have been reported to give acceptable results.
Does peripheral blood need to be diluted prior to loading into the Accuspin tubes (Product A7054)?
No.  The blood may either be used without dilution or with a 1:2 dilution.   In house studies at Sigma Aldrich have showed no decrease in recovery for peripheral blood specimens loaded directly into the Accuspin tubes.
May buffy coats or donor packs with reduced red cell numbers be used with Accuspin tubes (Product A7054)?
No.  For the mononuclear cells to band above the "frit", the specimen must contain a sufficient number of red cells to displace the density gradient above the "frit"
Can granulocytes be collected using the Accuspin tubes (Product A7054)?
No.  After centrifugation, the granulocytes will end up at the bottom of the Accuspin tube - just above the packed red blood cells.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Show more questions
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?