ACCUSPIN™ System-Histopaque®-1077
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MDL number MFCD01092091
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Properties
| Related Categories | Accuspin, Cell Biology, Density Gradient Media, General Stem Cell Biology, Gradients, |
| sterility | aseptically filled |
| shelf life | (Expiry date on the label.) |
| packaging | tube of 12 |
| storage temp. | 2-8°C |
Description
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Application
ACCUSPIN System-HISTOPAQUE-1077 employs centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit"). The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.
General description
γ-irradiated 50 mL polypropylene tubes fitted with a HDPE porous barrier and aseptically filled with 15 mL of Histopaque 1077. Each tube will separate 15-30 mL of anticoagulated blood.
Quantity
Kit contains 12 tubes.
Legal Information
Accuspin is a trademark of Sigma-Aldrich Co. LLC
Histopaque is a registered trademark of Sigma-Aldrich Co. LLC
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