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  • A7058 - Monoclonal Anti-polyHistidine−Peroxidase antibody produced in mouse

A7058 Sigma

Monoclonal Anti-polyHistidine−Peroxidase antibody produced in mouse

clone HIS-1, purified immunoglobulin, lyophilized powder

Synonym: Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal Anti-polyHistidine, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag



Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, HF-HI,
biological source   mouse
antibody form   purified immunoglobulin
clone   HIS-1, monoclonal
form   lyophilized powder
packaging   vial of 0.5 mL
concentration   5-11 mg/mL
application(s)   western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
isotype   IgG2a
conjugate   peroxidase conjugate
storage temp.   2-8°C



Antibody suitable for immunoblotting. Working dilution 1:2000
Also suitable for dot blot assays and ELISA

Features and Benefits

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General description

The monoclonal anti-polyHistidine Peroxidase Conjugate antibody recognizes native and denatured forms of synthetic polyhistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.


recombinant polyHistidine tagged fusion protein.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.


The antibody conjugate should be reconstituted with 0.5 ml deionized water.


The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal

Price and Availability

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles

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