• USA Home
  • A7058 - Monoclonal Anti-polyHistidine−Peroxidase antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
A7058 Sigma

Monoclonal Anti-polyHistidine−Peroxidase antibody produced in mouse

clone HIS-1, purified immunoglobulin, lyophilized powder

Synonym: Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal Anti-polyHistidine, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, HF-HI,
biological source   mouse
antibody form   purified immunoglobulin
clone   HIS-1, monoclonal
form   lyophilized powder
packaging   vial of 0.5 mL
concentration   5-11 mg/mL
application(s)   western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
isotype   IgG2a
conjugate   peroxidase conjugate
storage temp.   2-8°C

Description

Application

Antibody suitable for immunoblotting. Working dilution 1:2000
Also suitable for dot blot assays and ELISA

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

General description

The monoclonal anti-polyHistidine Peroxidase Conjugate antibody recognizes native and denatured forms of synthetic polyhistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.

Immunogen

recombinant polyHistidine tagged fusion protein.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

Reconstitution

The antibody conjugate should be reconstituted with 0.5 ml deionized water.

Specificity

The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal

Price and Availability


Antibody Explorer

Explore with Confidence

Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?