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A7080 Sigma

Amphiregulin human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)

Synonym: AR, AREG, CRDGF, SDGF

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Properties

Related Categories Amphiregulin, Cancer Research, Cell Biology, Cell Signaling and Neuroscience, Cytokines & Growth Factors,
recombinant   expressed in E. coli
assay   ≥97% (SDS-PAGE)
form   lyophilized powder
potency   10-100 ng/mL ED50/EC50
mol wt   mol wt ~11 kDa
impurities   endotoxin, tested
suitability   suitable for cell culture
storage temp.   −20°C
Gene Information   human ... AREG(374)

Description

Components

Human amphiregulin is produced by a DNA sequence that encodes a 98 amino acid residue form of mature human amphiregulin. This corresponds to amino acid residues 101-198 in E. coli. Amphiregulin is a glycoprotein produced in response to treatment of the cell line MCF-7, a human breast carcinoma cell line, with PMA, a protein kinase C activator and potent tumor promoter.

Analysis Note

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is typically 5-15 ng/mL.

Biochem/physiol Actions

Also known as keratinocyte autocrine factor (KAF); expression of amphiregulin mRNA has been found in numerous carcinoma cell lines and epithelial cells of several human tissues including colon, stomach, breast, ovary, and kidney.

Caution

This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.

Preparation Note

It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 10 μg/mL.

Physical form

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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