EMAIL THIS PAGE TO A FRIEND
A7089 Sigma

Accumax solution

sterile-filtered, suitable for cell culture

Purchase

Properties

Related Categories Cell Culture, Cell Dissociation, Cell Dissociation Enzymes, Reagents and Supplements
sterility   sterile-filtered
pH   6.8-7.8
suitability   suitable for cell culture
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Accumax has proven effective in dissociating clumped cells in suspension cultures of hybridomas, CHO, BHK, 293, COS and Sf9 cells. The solution performed extremely well in dissociating cell counting, viral transfection assays, cell sorting, flow cytometry, and bioreactor scale-up. It has also been used to create single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue, and the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.

Biochem/physiol Actions

Accumax is a cell detachment solution consisting of proteolytic and collagenolytic enzymes. Each bottle has 100 mL of 1x Accumax enzymes in Dulbecco′s PBS. There are no mammalian or bacterial-derived products found in Accumax.

Caution

Accumax is stable when stored at -20°C. After thawing, the product can be stored for up to 2 months at 4°C.

Features and Benefits

Ready-to-use sterile liquid for in vitro cell applications

Formula variant

Prepared in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4). Refer to Product Data Sheet on the website for product usage information.

Preparation Note

This product is supplied as a sterile, ready-to-use frozen liquid. Accumax should be thawed at 37°C before use, and added at equal volume to a sample of clumped cells. Allow 5-10 minutes at 37°C for incubation and count cells as you would normally.

Legal Information

Accumax is a trademark of Innovative Cell Technologies, Inc.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the appropriate cell dissociating product to be used for ES cells that will be injected into blastocysts?
Accutase®, product A6964, or Accumax™ (Accutase® with DNase), product A7089, will work well for dissociation of ES cells.  The Accutase® product has protease and dispase activity to dissociate the cells.  The Accumax™ product also has DNase to limit the aggregation that is caused by DNA released from ruptured cells during the dissociation/scraping. Please see the reference and link below for more information on the use of Accutase® with ES cells: Mol Reprod Dev. 2008 May;75(5):818-27 - http://innovativecelltech.com/Using%20Accutase%20to%20passage%20hESCs.pdf
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
Show more questions
Protocols & Articles

Articles

Stem Cell Basics

Stem cells have the unique ability to self-renew or to differentiate into various cell types in response to appropriate signals within the body. These properties provide stem cells with unique capabi...
Keywords: AGE, Apoptosis, Cancer, Cardiovascular, Cell biology, Clinical, Diabetes, Diagnostic, Diseases, Environmental, Genetic, Genetics, Industries, Inflammation, Pharmaceutical, Stem cell biology, Transcription, Transfection

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?