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A7089 Sigma

Accumax solution

sterile-filtered, suitable for cell culture

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Properties

Related Categories Cell Culture, Cell Dissociation, Cell Dissociation Enzymes, Reagents and Supplements
sterility   sterile-filtered
pH   6.8-7.8
suitability   suitable for cell culture
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Accumax has proven effective in dissociating clumped cells in suspension cultures of hybridomas, CHO, BHK, 293, COS and Sf9 cells. The solution performed extremely well in dissociating cell counting, viral transfection assays, cell sorting, flow cytometry, and bioreactor scale-up. It has also been used to create single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue, and the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.

Biochem/physiol Actions

Accumax is a cell detachment solution consisting of proteolytic and collagenolytic enzymes. Each bottle has 100 mL of 1x Accumax enzymes in Dulbecco′s PBS. There are no mammalian or bacterial-derived products found in Accumax.

Caution

Accumax is stable when stored at -20°C. After thawing, the product can be stored for up to 2 months at 4°C.

Features and Benefits

Ready-to-use sterile liquid for in vitro cell applications

Formula variant

Prepared in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4). Refer to Product Data Sheet on the website for product usage information.

Preparation Note

This product is supplied as a sterile, ready-to-use frozen liquid. Accumax should be thawed at 37°C before use, and added at equal volume to a sample of clumped cells. Allow 5-10 minutes at 37°C for incubation and count cells as you would normally.

Legal Information

Accumax is a trademark of Innovative Cell Technologies, Inc.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Stem Cell Basics

Stem cells have the unique ability to self-renew or to differentiate into various cell types in response to appropriate signals within the body. These properties provide stem cells with unique capabi...
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