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  • A7164 - Anti-Human Kappa Light Chains (Bound and Free)−Peroxidase antibody produced in goat

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A7164 Sigma

Anti-Human Kappa Light Chains (Bound and Free)−Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Human Kappa Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies,
application(s)   direct ELISA: 1:1,000
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... IGK@(50802)
biological source   goat
conjugate   peroxidase conjugate

Description

Immunogen

Purified human κ light chains

General description

Human κ light chains are antibody fragments that regulate immunological responses in cells. Increased expression of κ light chains has been linked to chronic lymphocytic leukemia . The variable fragment of the κ light chain gene has also been associated with vaccine-induced antibody response to the polysaccharide coat in Haemophilus influenzae type b . Anti-Human κ Light Chains (Bound and Free)-Peroxidase antibody detects human κ (bound and free) light chains when tested against human IgA, IgG, IgM, and Bence Jones κ and λ myeloma proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 0.01% thimerosal

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, p215, (Elsevier/North Holland Biomedical Press, Amsterdam, 1978).

Application

Anti-Human κ Light Chains (Bound and Free)-Peroxidase antibody is suitable for use in western blot (1:1000).

ELISA Assays were performed using HRP-conjugated goat anti-human kappa light chains IgG to detect IgG1 and free K chains in cultured media of human LCL cells.

Packaging

1 mL in glass bottle

Price and Availability


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