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A7294 Sigma

Avidin–Alkaline Phosphatase

buffered aqueous solution



Related Categories Antibodies, Avidin Reagents, Biotin/Avidin, Supplementary Products
biological source   alkaline phosphatase from calf intestine
  avidin from egg white
form   buffered aqueous solution
application(s)   direct ELISA: 1:70,000
  western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C


Preparation Note

Affinity purified protein conjugated to alkaline phosphatase using 0.2% glutaraldehyde.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.


Avidin-Alkaline Phosphatase has been used for ELISA. The product can also be used for western blot at 1:150,000-1:300,000 dilutions.

Cytokine ELISA Assays were performed using a biotinylated anti-IL-2 antibody and alkaline phosphatase avidin.

General description

Avidin is homotetrameric protein (68kDa) obtained from egg whites and binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

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Safety Information

NONH for all modes of transport
WGK Germany 
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Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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