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A7340 Sigma

Anti-Glutathione-S-Transferase (GST)–Peroxidase Conjugate antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Glutathione-S-Transferase, Anti-GST

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, Antibody Conjugates,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
mol wt   antigen mol wt 27.5 kDa
application(s)   direct ELISA: 1:10,000
  western blot: 1:10,000 using lysates of Escherichia coli induced to express recombinant GST
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

recombinant GST from Schistosoma japonicum expressed in E. coli.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal.

Application

N-terminally expressed GST-tagged proteins were over expressed and purified from E. coli. Purified proteins were analyzed by western blot using HRP-conjugated rabbit anti-GST antibody at a concentration of 20ng/ml.

Rabbit Anti-Glutathione-S-Transferase (GST)-Peroxidase Conjugate antibody has been used for western blot analaysis at a dilution of 1:500. The antibody can also be used for direct ELISA at 1:10,000.

General description

Recombinant target proteins are often expressed as a fusion product with Glutathione-S-Transferase (GST) tags using various expression vector constructs. Thus, antibodies directed against the GST tags of the recombinant constructs can facilitate the purification and study of target proteins.

The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

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