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A7420 Sigma

Amyloglucosidase from Aspergillus niger Green Alternative

lyophilized powder, 30-60 units/mg protein (biuret), ≤0.02% glucose

Synonym: 1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Amylases, α-Glucosidase, Starches (Amylose and Amylopectin) and Glycogen, Amyloglucosidase, Application Index,
form   lyophilized powder
composition   protein, ≥80%
greener alternative product characteristics   Waste Prevention: Greener alternative product characteristics
Learn more about the Principles of Green Chemistry.
impurities   ≤0.02% glucose
greener alternative category   Enabling
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

General description

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

25, 100 mg in poly bottle

5 mg in glass bottle

Unit Definition

One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.

Physical form

Lyophilized powder containing less than 0.02% glucose

Application

Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle .

Biochem/physiol Actions

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the molecular weight of Product A7420,  Amyloglucosidase from Aspergillus niger?
Amyloglucosidase from A. niger exists in two isoforms with MWs of 69,810 and 89,130, as determined by SDS-PAGE. See: Starch, 34, 346 (1982).
Does Product A7420,  Amyloglucosidase from Aspergillus niger, contain any stabilizers or additives?
The starting material for Product No. A7420 is Product No. A3042, which is a preparation of the enzyme in a 1 M glucose solution. This is dialyzed against water until the glucose concentration is less than 0.02%, and then lyophilized.
How can a solution of Product A7420,  Amyloglucosidase from Aspergillus niger, be prepared?
A 1 mg/mL solution may be prepared in water.
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Protocols & Articles

Protocols

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate. The Spectrophotometric Stop Rate Determination [Absorbance at 340 nm (A340), Light path = ...
Keywords: Enzyme activity, Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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