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A7437 Sigma

Acetate Kinase from Escherichia coli

lyophilized powder, ≥150 units/mg protein (biuret)

Synonym: ATP:Acetate phosphotransferase

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Properties

Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   lyophilized powder
composition   Protein, 5.0-20.0%
foreign activity   adenosine triphosphatase and NADH oxidase ≤0.01%
storage temp.   −20°C
Gene Information   Escherichia coli K12 ... ackA(946775)

Description

Unit Definition

One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.6 at 25 °C.

Physical form

Lyophilized powder containing trehalose with small amounts of potassium phosphate, magnesium chloride, and dithiothreitol

Application

Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Escherichia coli has been used as part of an ATP-regenerating system to study the kinetics of agonist-stimulated transphosphatidylation .

Biochem/physiol Actions

Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA . Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine.

Involved in the metabolism of propanoate, pyruvate and taurine

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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