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  • A7549 - Anti-Apoptosis-Inducing Factor antibody produced in rabbit

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A7549 Sigma

Anti-Apoptosis-Inducing Factor antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-AIF

Properties

Related Categories AO-AQ, Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology,
species reactivity   human, mouse
application(s)   microarray: suitable
  western blot: 1:1,000 using human epitheloid carcinoma HeLa cell extract and mouse brain extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 57 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... AIFM1(9131)
mouse ... Aifm1(26926)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminus of human Apoptosis Inducing Factor (AIF), (amino acids 593-613), conjugated to KLH.

General description

Apoptosis-Inducing Factor (AIF) is a mitochondrial flavoprotein that can induce apoptosis in isolated nuclei. Studies have reported that AIF can induce the realease of cytochrome c and caspase 9. Bcl-2 is known to inhibit AIF release without affecting its apoptotic functions.
Rabbit Anti-AIF antibody recognizes human and mouse AIF (57kDa). Staining of AIF in immunoblotting is specifically inhibited with the AIF immunizing peptide (human, amino acids 593-613).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Protein lysates from 2CLL, 10 MCl and Jeko-1 cells were analyzed by western blot using anti-AIF as the primary antibody. Cells also underwent intracellular delivery by being transfected with anti-AIF at a final dilution of 1:2500 and then analyzed by FLOW cytometry.

Rabbit Anti-Apoptosis-Inducing Factor (AIF) antibody has been used for immunoblot analysis at 1:1000 and 1:2000 dilutions. The antibody can also be used for microarray applications.

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