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  • A7554 - Monoclonal Anti-Bovine IgG−Alkaline Phosphatase antibody produced in mouse

A7554 Sigma

Monoclonal Anti-Bovine IgG−Alkaline Phosphatase antibody produced in mouse

clone BG-18, purified immunoglobulin, buffered aqueous glycerol solution

Synonym: Monoclonal Anti-Bovine IgG



Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, BL-BZ, Bovine Secondary Antibodies and Conjugates,
biological source   mouse
antibody form   purified immunoglobulin
clone   BG-18, monoclonal
form   buffered aqueous glycerol solution
species reactivity   bovine
does not react with   guinea pig, goat, pig, sheep, bovine (IgM), cat, horse, rabbit, canine, human
application(s)   direct ELISA: 1:25,000
  dot blot: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG
  western blot (chemiluminescent): 1:100,000
isotype   IgG1
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C



Purified bovine IgG


bovine IgG1 and IgG2

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide


P.haemolytica cell lysates were anlyzed by western blot using alkaline phosphatase-conjugated monoclonal mouse anti-bovine IgG as the secondary at a dilution of 1:20000 in TSGT.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse monoclonal Anti-Bovine IgG-Alkaline Phosphatase antibody can be used for ELISA and immunoblot applications. The antibody can also be used for dot blot and immunohistochemistry (1:75) assays.

General description

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

No cross-reaction is observed with bovine IgM or with IgG derived from the following species: human, goat, sheep, rabbit, pig, dog, checken, cat, guinea pig, or horse.

The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction.

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Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


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Secondary Antibodies, Conjugates and Kits

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Peer-Reviewed Papers


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