A7646 Sigma

Apyrase from potatoes

ATPase 40-200 units/mg protein, lyophilized powder (partially purified)

Synonym: Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

Properties

Related Categories 3.6.x.x Acting on acid anhydrides, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
type   Grade III
form   lyophilized powder (partially purified)
composition   Protein, 30-60%
solubility   H2O: soluble10 mg/mL, slightly hazy
Market Segment   Diagnostic Assay Manufacturing
foreign activity   Acid Phosphatase ≤5% of base activity
storage temp.   −20°C

Description

Application

At least two isoenzymes are found in different varieties of S. tuberosum:5,6 one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.11 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation.2 The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation.3

Other Notes

Mixture of high and low ratio isoenzymes.

Packaging

Sold on the basis of ATPase units

Quality

Contains ≤ 5% acid phosphatase.

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis.4 Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa.

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