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  • A7650 - Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

A7650 Sigma

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution



Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, GM-GO, Goat Secondary Antibodies and Conjugates,
biological source   rabbit
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:7,000
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C


Physical form

Solution in 0.05 M Tris, pH 8.0, containing 10 mM glycine, 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.


Immunocytochemistry was performed on cells fixed in 4% paraformaldhyde for 15 minutes using alkaline phosphatase-conjugated rabbit anti-goat IgG diluted 1:1000 in TBS as the secondary antibody.

General description

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antiserum is determined to be immunospecific for goat IgG by immunoelectrophoresis (IEP), prior to conjugation, versus goat IgG and normal goat serum.

Second antibodies (secondary antibodies) are antibodies raised against other antibodies, typically from another species, called primary antibodies. Primary antibodies are generated with and bind to specific antigens to create antigen antibody complexes which may be detected in a variety of immunochemical and immunohistochemical configurations involving soluble or insoluble signaling molecules. The immunodetection system depends upon the binding of a signal competent secondary antibody to a primary antibody:antigen complex.
The suitability of any secondary antibody to a specific immunodetection application is dependent upon its signal development system configuration. Signal development systems are based upon the chemical conjugation of a signal mediator to the secondary antibody. Major secondary antibody signal systems are color (chromogenic), chemiluminescence or fluorescence based and involve the use of enzymes or high affinity binary systems such as biotin:avidin.
Alkaline Phosphatase is an enzyme that catalyzes the conversion of chromogenic substrates such as p-nitrophenylphosphate (PNPP); chemiluminescent substrates such as CDP-Star and fluorogenic substrates such as 4-methylumbelliferyl phosphate (4-MUP) into detectable chromophores, light-emitters or fluorescers, respectively.

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