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A7674 Sigma

Anti-AOP1 antibody, Mouse monoclonal

clone AOP-38, purified from hybridoma cell culture

Synonym: Anti-Antioxidant-like Protein 1, Anti-PRDX3, Anti-Peroxiredoxin 3

Properties

Related Categories AO-AQ, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress,
species reactivity   hamster, chicken, monkey, mouse, human, rat, canine
application(s)   indirect ELISA: suitable
  microarray: suitable
  western blot: 0.25-0.5 μg/mL using a whole extract of cultured human acute T cell leukemia, Jurkat, cells
clone   AOP-38, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 25 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PRDX3(10935)
mouse ... Prdx3(11757)
rat ... Prdx3(64371)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to C-terminal amino acids 236-256 of human Aop1, conjugated to KLH.

General description

Antioxidant-like Protein 1 (AOP1) belongs to a protein family that may regulate oxidative stress defense. AOP1 is also proposed to be involved in the synthesis of glucosinolate.
Monoclonal Anti-Aop1 (Antioxidant-like Protein 1) reacts specifically with Aop1 and does not recognize other proteins involved in defense of oxidative stress, such as alkylhydroperoxide reductase (AhpC), glutathion peroxidase and peroxiredoxin. The epitope recognized by the antibody resides within the C-terminal amino acids 236-256 of human Aop1. The antibody binds to human, monkey, dog, hamster, rat, mouse and chicken Aop1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

AOP1 expression in cells isolated from human saliva was analyzed by western blot using mouse monoclonal anti-AOP1 antibody (A7674) at a 1:10000 dilution.

Mouse monoclonal anti-AOP1 antibody can be used for indirect ELISA and microarray applications.

Price and Availability


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