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A7721 Sigma

JumpStart Taq Antibody

Adds hot-start capabilities to any Taq DNA Polymerase



Related Categories Alphabetical Index, Antibodies, Hot Start PCR, J, Molecular Biology,
form   liquid
feature   hotstart
concentration   1.1 mg/mL
color   colorless
conjugate   unconjugated
shipped in   wet ice
storage temp.   −20°C



JumpStart Taq Antibody, 1.1μg/1μL
Dilution Buffer provided in 1 mL vials


The primary purpose of all hot start PCR methods is to prevent Taq DNA polymerase activity prior to thermal cycling. Even if set-up is conducted on ice, the Taq DNA polymerase remains active and may elongate unwanted products such as misprimed or other non-specific events. Primer-dimer interactions may also be amplified, which will reduce overall yield and efficiency of desired products.

One way to prevent unwanted amplification products is to add JumpStart Taq Antibody to the reaction. This efficient yet simple procedure takes only 10 minutes and effectively inactivates the Taq DNA polymerase until the first denaturation cycle. Upon heating to 70 °C, the antibody dissociates and full activity is restored to the Taq DNA polymerase for the remainder of the PCR. Unlike chemically inactivated hot-start methods, no extended heating step is required for reactivation with the use of JumpStart Taq Antibody.

JumpStart Taq antibody works effectively on a variety of commercially available Taq DNA polymerases.

Features and Benefits

• Minimize non-specific amplification while increasing target yield & specificity
• Reduce set-up time and eliminate concerns associated with manual or wax hot-start methods.

Other Notes

View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.


Supplied with dilution buffer.


Two units of Taq DNA polymerase are inactivated by 1 test of JumpStart Taq Antibody.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

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