• USA Home
  • A7721 - JumpStart Taq Antibody

EMAIL THIS PAGE TO A FRIEND
A7721 Sigma

JumpStart Taq Antibody

Adds hot-start capabilities to any Taq DNA Polymerase

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Hot Start PCR, J, Molecular Biology,
form   liquid
feature   hotstart
concentration   1.1 mg/mL
color   colorless
conjugate   unconjugated
shipped in   wet ice
storage temp.   −20°C

Description

Components

JumpStart Taq Antibody, 1.1μg/1μL
Dilution Buffer provided in 1 mL vials

Application

The primary purpose of all hot start PCR methods is to prevent Taq DNA polymerase activity prior to thermal cycling. Even if set-up is conducted on ice, the Taq DNA polymerase remains active and may elongate unwanted products such as misprimed or other non-specific events. Primer-dimer interactions may also be amplified, which will reduce overall yield and efficiency of desired products.

One way to prevent unwanted amplification products is to add JumpStart Taq Antibody to the reaction. This efficient yet simple procedure takes only 10 minutes and effectively inactivates the Taq DNA polymerase until the first denaturation cycle. Upon heating to 70 °C, the antibody dissociates and full activity is restored to the Taq DNA polymerase for the remainder of the PCR. Unlike chemically inactivated hot-start methods, no extended heating step is required for reactivation with the use of JumpStart Taq Antibody.

JumpStart Taq antibody works effectively on a variety of commercially available Taq DNA polymerases.

Features and Benefits

• Minimize non-specific amplification while increasing target yield & specificity
• Reduce set-up time and eliminate concerns associated with manual or wax hot-start methods.

Other Notes

View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Packaging

Supplied with dilution buffer.

Quantity

Two units of Taq DNA polymerase are inactivated by 1 test of JumpStart Taq Antibody.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


Detect Your Unseen Protein

All labs need water
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?