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  • A7732 - Anti-α-Actinin (Sarcomeric) antibody, Mouse monoclonal

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A7732 Sigma

Anti-α-Actinin (Sarcomeric) antibody, Mouse monoclonal

clone EA-53, purified from hybridoma cell culture

Synonym: Anti-1110008F24Rik, Anti-ACTN2, Anti-Actinin α2, Anti-CMD1AA, Anti-MGC107582

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin,
species reactivity   human, mouse, pig, rabbit, bovine, rat, snake, frog, cat, fish, canine, goat, chicken, sheep, hamster, lizard
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using protease-digested sections of human tongue.
  western blot: 2-4 μg/mL using rat skeletal muscle extract
clone   EA-53, monoclonal
concentration   ~1.0 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACTN3(88)
mouse ... Actn2(11472)
rabbit ... ACTN3(100009544)
rat ... Actn2(291245)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

purified rabbit skeletal α actinin.

General description

Alpha-actinin is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. In smooth muscles, α-actinin is present in dense bodies and plaques whereas in normal skeletal muscles, it is associated with z-discs that define muscle sarcomeres.
Monoclonal Anti-α-Actinin (Sarcomeric) shows wide reactivity, as it binds to human, bovine, pig, sheep, rabbit, goat, hamster, cat, rat, mouse, dog, chicken, lizard, snake, frog and fish α-actinin.

Monoclonal Anti-α-Actinin (Sarcomeric) specifically recognizes α-skeletal muscle actinin and α-cardiac muscle actinin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunofluorescence (2 papers)
Immunohistochemistry (1 paper)

Mouse Monoclonal Anti-alpha-Actinin (Sarcomeric) antibody has been used for immunocytochemical (1:800) and immunohistochemical (1:100) applications.

Western blot analysis of transfected HEK-293 cells were performed to evaluate actinin expression using monoclonal mouse anti-actinin antibody.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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