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  • A7811 - Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse

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A7811 Sigma

Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse

clone EA-53, ascites fluid

Synonym: Anti-1110008F24Rik, Anti-ACTN2, Anti-Actinin α2, Anti-CMD1AA, Anti-MGC107582

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin,
species reactivity   sheep, rabbit, lizard, feline, snake, canine, fish, human, mouse, goat, chicken, rat, frog, bovine, pig, hamster
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800 using human skeletal and cardiac muscle
  indirect ELISA: suitable using human and animal muscle tissue
  indirect immunofluorescence: suitable using human and animal cultured muscle cells
  western blot: 1:2,500 using rat leg muscle
clone   EA-53, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 100 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACTN2(88)
mouse ... Actn2(11472)
rat ... Actn2(291245)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

rabbit skeletal α-actinin

General description

α-Actinin is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. In smooth muscles, α-actinin is present in dense bodies and plaques whereas in normal skeletal muscles, it is associated with z-discs that define muscle sarcomeres.
Mouse monoclonal anti-α-actinin (sarcomeric) antibody stains thymic myoid cells. The antibody exhibits wide reactivity with human and animal muscle tissue.

The antibody is specific for α-skeletal and α-cardiac muscle actinins. The antibody labels Z lines and dots in stress fibers of skeletal muscle in myotubes but does not react with non-skeletal muscle elements (e.g., connective tissue, epithelium, nerves, smooth muscle).

Application

Cardiac myocytes were stained with monoclonal mouse anti-actinin to visualize the Z-lines after fixation in pre-cooled methanol/acetone mix (1:1) for 10 minutes at 4 °C.

Mouse monoclonal anti-α-actinin (sarcomeric) antibody is suitable for immunohistochemistry (1:800), ELISA, immunofluorescence and western blot (1:2,500) applications.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

Articles

Fluorescent Live Cell Imaging of Cytoskeleton Structure Proteins using LentiBrite™ Lentiviral Biosensors

Cell analysis of the dynamics of subcellular structures has been revolutionized in the past 15 years by the development and refinement of genetically-encoded fusions between fluorescent proteins (GFP...
Keywords: Apoptosis, Cloning, Fluorescent microscopy, Gene expression, Immunocytochemistry, Immunofluorescence, Microscopy, Rearrangements, Tagged proteins, Transduction, Transfection

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Peer-Reviewed Papers
15

References

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Description

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A7732 Anti-α-Actinin (Sarcomeric) antibody, Mouse monoclonal, clone EA-53, purified from hybridoma cell culture
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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