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  • A7811 - Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse

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A7811 Sigma

Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse

clone EA-53, ascites fluid

Synonym: Anti-1110008F24Rik, Anti-ACTN2, Anti-Actinin α2, Anti-CMD1AA, Anti-MGC107582

Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin,
species reactivity   sheep, rabbit, lizard, feline, canine, snake, human, fish, goat, mouse, chicken, rat, bovine, frog, pig, hamster
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800 using human skeletal and cardiac muscle
  indirect ELISA: suitable using human and animal muscle tissue
  indirect immunofluorescence: suitable using human and animal cultured muscle cells
  western blot: 1:2,500 using rat leg muscle
clone   EA-53, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 100 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACTN2(88)
mouse ... Actn2(11472)
rat ... Actn2(291245)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

rabbit skeletal α-actinin

General description

α-Actinin is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. In smooth muscles, α-actinin is present in dense bodies and plaques whereas in normal skeletal muscles, it is associated with z-discs that define muscle sarcomeres.
Mouse monoclonal anti-α-actinin (sarcomeric) antibody stains thymic myoid cells. The antibody exhibits wide reactivity with human and animal muscle tissue.

The antibody is specific for α-skeletal and α-cardiac muscle actinins. The antibody labels Z lines and dots in stress fibers of skeletal muscle in myotubes but does not react with non-skeletal muscle elements (e.g., connective tissue, epithelium, nerves, smooth muscle).

Application

Cardiac myocytes were stained with monoclonal mouse anti-actinin to visualize the Z-lines after fixation in pre-cooled methanol/acetone mix (1:1) for 10 minutes at 4 °C.

Mouse monoclonal anti-α-actinin (sarcomeric) antibody is suitable for immunohistochemistry (1:800), ELISA, immunofluorescence and western blot (1:2,500) applications.

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