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A7827 Sigma

Alkaline Phosphatase-Anti-Alkaline Phosphatase antibody produced in mouse

clone AP1B9, purified immunoglobulin, buffered aqueous solution

Synonym: APAAP

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Properties

Related Categories AL-AL, APAAP, Alphabetical Index, Antibodies, Primary Antibodies,
biological source   mouse
antibody form   purified immunoglobulin
clone   AP1B9, monoclonal
description   Concentrate
form   buffered aqueous solution
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil
isotype   IgG1
shipped in   wet ice
storage temp.   2-8°C

Description

Physical form

Solution in 0.05 M Tris buffered saline, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin and 15 mM sodium azide.

Application

Apoptosis of β-cells in mouse pancreatic sections was analyzed by incubating section with TUNEL and anti-insulin antibody. APAAP was used as the secondary antibody and BCIP/NBT (Sigma) as the substrate.

General description

A soluble complex of calf intestinal alkaline phosphatase and mouse monoclonal antibodies to alkaline phosphatase. The product is a soluble complex of calf intestinal alkaline phosphatase and mouse monoclonal antibodies to alkaline phosphatase.

May be used in immunohistology and immunocytology using primary mouse antibodies, bridging antibody, mouse APAAP and substrate.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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