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A7981 Sigma

Anti-Aromatase antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-ARO, Anti-CYP XIX, Anti-CYP19A1 (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1), Anti-Estrogen Synthase, Anti-P-450 Aromatase

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Properties

Related Categories AR-AR, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Multi-Drug Resistance,
species reactivity   human
application(s)   indirect immunofluorescence: 0.5-1 μg/mL using paraformaldehyde fixed HEK-293T cells transfected with Aromatase
  western blot: 0.5-1 μg/mL using human placenta tissue lysate
clone   polyclonal
concentration   ~1.0 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 50-55 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CYP19A1(1588)
biological source   rabbit
conjugate   unconjugated

Description

General description

The enzyme aromatase is a member of P450 cytochrome family. Aromatase catalyzes three oxidation reactions of androstenedione A that lead to aromatization of androgens and subsequent synthesis of estrogen. The enzymatic complex of aromatase is a heterocomplex of cytochrome 450 aromatase and NADPH cytochrome P450 reductase. The tissues that produce aromatase are placenta, ovary, brain, bone, adipose tissue, skin and testis. The aromatase enzyme complex is present in the smooth endoplasmic reticulum of cells of these tissues. High levels of aromatase and estrogen are produced by the breast cancer cells. Selective aromatase inhibitors are developed to control the recurrence and survival of hormone-dependent breast cancer tissue
Anti-Aromatase specifically recognizes human aromatase (50-55 kDa).

Antigen Background

Aromatase is part of the cytochrome p450 family of monooxygenases. It is involved in the production of estrogen and found localized to the endoplasmic reticulum of multiple cell types.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-Aromatase may be used for immunoblotting at a working concentration of 0.5-1 μg/ml using lysate of human placenta tissue. The antibody has also been used for immunoblotting using whole extracts or nuclear extracts of human primary trophoblasts. For immunofluorescence, a working concentration of 0.5-1 μg/ml is recommended.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

Articles

Phase I Drug Metabolism

Phase I biotransformation reactions introduce or expose functional groups on the drug with the goal of increasing the polarity of the compound. Although Phase I drug metabolism occurs in most tissues...
Robert Gates
Biofiles Volume 6 Article 1
Keywords: Catalysis, Eliminations, Environmental, Enzyme-linked immunosorbent assay, Gastrointestinal, Gene expression, Immunohistochemistry, Metabolism, Metabolites, Oxidations, Post translational modifications, Reductions, Support, Western blot

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Peer-Reviewed Papers
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References

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