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A8181 Sigma

Amido Black Staining Solution 2X

electrophoresis reagent

Synonym: Amido Black 10B, Naphthol Blue Black solution

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Description

Application

Naphthol Blue Black stain is designed for rapid staining of protein bands on nitrocellulose membranes (Western blots). Blots are stained for 1 min and then destained for 30 minutes in 25% (v/v) isopropanol, 10% (v/v) acetic acid. Used to detect proteins in the lower microgram range with a clear background. Naphthol Blue Black can also be used for staining PAGE gels.

Reconstitution

After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN 2924 8(3) / PGIII
WGK Germany 
2
Flash Point(F) 
86 °F
Flash Point(C) 
30 °C
Protocols & Articles

Articles

Electrophoresis - Protein Staining Reagents

To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, als...
Keywords: PAGE, Proteomics

Peer-Reviewed Papers
15

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