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A8273 Sigma

Anti-α-Amylase antibody produced in rabbit

fractionated antiserum, lyophilized powder

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Properties

Related Categories AM-AN, Alphabetical Index, Antibodies, Miscellaneous, Primary Antibodies More...
species reactivity   human
application(s)   Ouchterlony double diffusion: suitable
  indirect ELISA: 1:4,000-1:6,000
clone   polyclonal
antibody form   fractionated antiserum
form   lyophilized powder
storage temp.   2-8°C
Gene Information   human ... AMY1A(276)
biological source   rabbit
packaging   vial of 2 mL lyophilized antiserum
conjugate   unconjugated

Description

Immunogen

human salivary α-amylase.

General description

α amylase is an enzyme present in human saliva and pancreatic secretions which activates in the presence of a chloride ion and needs calcium ion to maintain its structural integrity. Anti-α-amylase antibody (diluted 1:200) can be used in immunostaining.It can also be used as primary antibody in immunohistochemistry. Anti-α-amylase antibody reacts specifically with α amylase present in human saliva and pancreatic extract but not with other proteins of saliva and pancreatic extract.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2

Specificity

The antiserum is specific for human a-amylase found in human saliva and human pancreatic extract. No reaction with other human saliva proteins or pancreatic extract proteins is observed.

Application

It can be used in immunofluorescence and immunohistochemical analysis.

Mouse pancreatic sections were fixed in 4% paraformaldehyde and used for immunohistochemistry using rabbit anti-amylase antibody at a dilution of 1:100.

Reconstitution

Reconstitute with 2 mL deionized water.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Peer-Reviewed Papers
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