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A8311 Sigma

Antifoam 204

aqueous emulsion for bacterial and mammalian systems

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Properties

Related Categories Antifoams, Core Bioreagents, General Reagents, Life Science Reagents for Transfection, Media Components,
form   emulsion (aqueous)
concentration   100% (organic)
density   1.01 g/mL at 25 °C
storage temp.   room temp
suitability   (Mammalian (suspension))
  Bacteria (fermentation)

Description

Components

Antifoam 204 is a 100% active components and a mixture of non-silicone organic defoamers in a polyol dispersion.

Application

Suitable for preventing or minimizing foaming in microbial fermentation systems and mammalian suspension cultures.

General description

Antifoam 204 is an organic antifoam used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Other Notes

Contains 100% active components and is a mixture of non-silicone organic defoamers in a polyol dispersion. Can be sterilized repeatedly.

Preparation Note

For use in microbiological media Sigma recommends a starting concentration of between 0.005% and 0.01%.
The optimal amount of antifoam required for various applications will need to be determined. Antifoam 204 is soluble in methanol, ethanol, toluene, xylene, perchloroethylene, and cold water at temperatures below 15 °C. It is insoluble in warm water and ethylene glycol.

Price and Availability

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antifoams

A6426 Antifoam 204 A8311 Antifoam 204 (autoclaved) A5633 Antifoam A Concentrate A6582 Antifoam A Concentrate Molecular biology grade A5757 Antifoam B Emulsion A8011 Antifoam C Emulsion A5758 Antifoam...
Keywords: Antimicrobials, Culture media, Fermentation, Filtration, Molecular biology, Nucleic acid denaturation, Sample preparations, Separation

Protocols

EnPresso® B 500 Animal-free Protocol

EnPresso® B is a pre-sterilized growth system designed to increase the yield of functional protein from E. coli-based expression systems. EnPresso growth systems provide optimal conditions for growth...
Keywords: Antibiotics, Diagnostic, Filtration, Gene expression, Metabolism, Vitamins

EnPresso® B 500 Protocol

EnPresso® B is a pre-sterilized growth system designed to increase the yield of functional protein from E.coli-based expression systems. EnPresso growth systems provide optimal conditions for growth,...
Keywords: Antibiotics, Diagnostic, Filtration, Gene expression, Metabolism, Vitamins

EnPresso® B Defined Nitrogen-free 500 Protocol

EnPresso® B Defined Nitrogen-free 500 is a pre-sterilized, chemically-defined growth system designed to increase the yield of 15N-labelled proteins.
Keywords: Antibiotics, Diagnostic, Filtration, Gene expression, Metabolism, Vitamins

Peer-Reviewed Papers
15

References

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