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  • A8400 - Anti-Human Polyvalent Immunoglobulins (G,A,M)−Peroxidase antibody produced in goat

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A8400 Sigma

Anti-Human Polyvalent Immunoglobulins (G,A,M)−Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Human Polyvalent Ig Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:5,000-1:15,000 using IgG, IgA, IgM
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Purified human IgA, IgG, and IgM

Packaging

2 mL in glass bottle

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 0.01% thimerosal

Application

Anti-Human Polyvalent Immunoglobulins (G,A,M)-Peroxidase antibody is suitable for use in ELISA and immunoblot.

ELISA was performed to determine serotype-specific antibody avidity indexes for sera and monoclonal antibodies using HRP-conjugated goat anti-human polyvalent IgG with incubation for 2 hours.

General description

Human immunoglobulins are glycoproteins that regulate immune responses to infections and allergies. Anti-human polyvalent immunoglobulins can be used for serological analyses in diseased population groups . These antibodies may also be used for studying xenotransplantation models and immunological disorders such as rheumatoid arthritis . Anti-Human Polyvalent Immunoglobulins (G,A,M)-Peroxidase antibody is specific for human IgG, IgA and IgM when tested against human IgA, IgG, IgM, and Bence Jones κ and λ myeloma proteins.

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Amplified Detection
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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