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A8419 Sigma

Anti-Human IgG (γ-chain specific)−Peroxidase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Human IgG Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies,
biological source   goat
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:40,000
  dot blot: 1:100,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Human IgG

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.01% thimerosal

Application

Anti-Human IgG (γ-chain specific)-Peroxidase antibody may be used in direct ELISA (1:40,000), immunohistochemistry (1:100 dilution in formalin-fixed, paraffin-embedded sections) and dot blot (1:100,000).

IgM concentration in sera from patients with chronic CA syndrome was determined by ELISA using HRP-conjugated goat anti-human (mu chain specific) as the secondary antibody.

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . The specificity of Anti-Human IgG (γ-chain specific)-Peroxidase antibody is determined by immunoelectrophoresis and Ouchterlony Double Diffusion techniques. This antibody is specific for for human IgG when tested against purified human IgA, IgG, IgM, Bence Jones κ and Bence Jones lambda myeloma proteins.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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