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A8531 Sigma

Bovine Serum Albumin

Synonym: Albumin bovine serum, BSA, Bovine albumin

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Description

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn2 method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Application

Albumin from bovine serum can be used as a gel filtration molecular weight marker. It can be used in medical studies to quantify binding capacity and adsorption rate of protein enrichment and separation medium critical in proteomic research.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.1

Protocols & Applications

Select the right albumin for your application with the Albumin Selection Guide

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Safety & Documentation

Safety Information

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3

Protocols & Articles

Peer-Reviewed Papers

References

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1. Characterization of bovine serum albumin epitopes and their role in allergic reactions Restani, P., et al. Allergy 59(Suppl. 78), 21-24, (2004)

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2. Preparation and properties of serum and plasma proteins; a system for the separation into fractions of the protein and lipoprotein components of biological tissues and fluids. Cohn, E.J., et al. J. Am. Chem. Soc. 68, 459, (1946)

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In vitro hepatic metabolism of 2,2',4,4',5-pentabromodiphenyl ether (BDE 99) in Chinook salmon (Onchorhynchus tshawytscha). Browne EP Aquat. Toxicol. 92(4), 281-7, (2009)

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Contact-dependent growth inhibition requires the essential outer membrane protein BamA (YaeT) as the receptor and the inner membrane transport protein AcrB. Stephanie K. Aoki et al Mol. Microbiol. 70, 323-40, (2008)

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Isolation of functional human endothelial cells from small volumes of umbilical cord blood. Kang SD, Carlon TA, Jantzen AE, et al. Ann. Biomed. Eng. 41(10), 2181-92, (2013)

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Mercury vapor release from broken compact fluorescent lamps and in situ capture by new nanomaterial sorbents. Johnson, NC Environ. Sci. Technol. 42(15), 5772-8, (2008)

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Identification of a bile-induced exopolysaccharide required for Salmonella biofilm formation on gallstone surfaces. Crawford RW Infect. Immun. 76(11), 5341-9, (2008)

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TLR4 signaling induced by lipopolysaccharide or paclitaxel regulates tumor survival and chemoresistance in ovarian cancer. Szajnik M Oncogene 28(49), 4353-63, (2009)

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Tumor-growth-promoting cyclooxygenase-2 prostaglandin E2 pathway provides medulloblastoma therapeutic targets. Baryawno N Neuro. Oncol. 10(5), 661-74, (2008)

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Interoceptive effects of alcohol require mGlu5 receptor activity in the nucleus accumbens. Besheer J J. Neurosci. 29(30), 9582-91, (2009)

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Administration of probiotics influences F4 (K88)-positive enterotoxigenic Escherichia coli attachment and intestinal cytokine expression in weaned pigs. Daudelin JF Vet. Res. 42(1), 69, (2011)

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Lentiviral vectors bearing the cardiac promoter of the Na+-Ca2+ exchanger report cardiogenic differentiation in stem cells. Barth AS Mol. Ther. 16(5), 957-64, (2008)

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Identification of a novel self-sufficient styrene monooxygenase from Rhodococcus opacus 1CP. Tischler D J. Bacteriol. 191(15), 4996-5009, (2009)

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Multiple functional domains of Enterococcus faecalis aggregation substance Asc10 contribute to endocarditis virulence. Olivia N. Chuang et al Infect. Immun. 77, 539-48, (2009)

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Quantitation of alpha-linolenic acid elongation to eicosapentaenoic and docosahexaenoic acid as affected by the ratio of n6/n3 fatty acids. Harnack K Nutr. Metab. 6, 8, (2009)

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Quantitative detection of small molecule/DNA complexes employing a force-based and label-free DNA-microarray. Dominik Ho et al Biophys. J. 96, 4661-71, (2009)

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The essential role of single Ig IL-1 receptor-related molecule/Toll IL-1R8 in regulation of Th2 immune response. Bulek K J. Immunol. 182(5), 2601-9, (2009)

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Competitive adsorption: a physical model for lung surfactant inactivation. Fernsler JG and Zasadzinski JA Langmuir 25(14), 8131-43, (2009)

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Threonine-5 at the N-terminus can modulate sarcolipin function in cardiac myocytes. Bhupathy P J. Mol. Cell. Cardiol. 47(5), 723-9, (2009)

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Modulation of interleukin-1β-induced inflammatory responses by a synthetic cationic innate defence regulator peptide, IDR-1002, in synovial fibroblasts. Turner-Brannen E Arthritis. Res. Ther. 13(4), R129, (2011)

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Histone H2AX phosphorylation in response to changes in chromatin structure induced by altered osmolarity. Baure J Mutagenesis 24(2), 161-7, (2009)

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Conjunctival interleukin-13 expression in mucous membrane pemphigoid and functional effects of interleukin-13 on conjunctival fibroblasts in vitro. Saw VP Am. J. Pathol. 175(6), 2406-15, (2009)

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Transplantation of adrenal cortical progenitor cells enriched by Nile red. Dunn JC, Chu Y, Qin HH, et al. J. Surg. Res. 156(2), 317-24, (2009)

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A mitotic GlcNAcylation/phosphorylation signaling complex alters the posttranslational state of the cytoskeletal protein vimentin. Slawson C Mol. Biol. Cell 19(10), 4130-40, (2008)

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Proteomic characterization of plasma membrane-proximal T cell activation responses. Ben de Wet et al J. Biol. Chem. 286, 4072-80, (2011)

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H5N1 avian influenza virus induces apoptotic cell death in mammalian airway epithelial cells. Tomo Daidoji et al J. Virol. 82, 11294-307, (2008)

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Characterization of the ompL1 gene of pathogenic Leptospira species in China and cross-immunogenicity of the OmpL1 protein. Dong H BMC Microbiol. 8, 223, (2008)

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Resolving phenylalanine metabolism sheds light on natural synthesis of penicillin G in Penicillium chrysogenum. Veiga T Eukaryotic Cell 11(2), 238-49, (2012)

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Inhibition of OXA-1 beta-lactamase by penems. Bethel CR Antimicrob. Agents Chemother. 52(9), 3135-43, (2008)

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Stimulation of neoplastic mouse lung cell proliferation by alveolar macrophage-derived, insulin-like growth factor-1 can be blocked by inhibiting MEK and PI3K activation. Fritz JM Mol. Cancer 10, 76, (2011)

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Diversity in genomic organisation, developmental regulation and distribution of the murine PR72/B" subunits of protein phosphatase 2A. Karen Zwaenepoel et al BMC Genomics 9, 393, (2008)

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Phase I study of a herpes simplex virus type 2 (HSV-2) DNA vaccine administered to healthy, HSV-2-seronegative adults by a needle-free injection system. Ashok Cattamanchi et al Clin. Vaccine Immunol. 15, 1638-43, (2008)

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Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening. Yu X Antimicrob. Agents Chemother. 53(10), 4311-9, (2009)

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Altered states of telomere deprotection and the two-stage mechanism of replicative aging. Zou Y Mol. Cell. Biol. 29(9), 2390-7, (2009)

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Mechanisms of confluence-dependent expression of CD26 in colon cancer cell lines. Abe, M., et al. BMC Cancer 11, 51, (2011)

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Expression of activated STAT5 in neoplastic mast cells in systemic mastocytosis: subcellular distribution and role of the transforming oncoprotein KIT D816V. Christian Baumgartner et al Am. J. Pathol. 175, 2416-29, (2009)

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Pharmacokinetic-pharmacodynamic modelling of fluvoxamine 5-HT transporter occupancy in rat frontal cortex. Geldof M Br. J. Pharmacol. 154(6), 1369-78, (2008)

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Cationic pyridinium porphyrins appending different peripheral substituents: Spectroscopic studies on their interactions with bovine serum albumin. Zhao, P., et al. Spectrochim. Acta A 88, 130-136, (2012)

Preparation of procion brilliant blue-doped silica nanorods and their recognition properties for proteins Deng, Q., et al. Se Pu 29, 876-880, (2011)

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Impact of protein binding on the analytical detectability and anticancer activity of thymoquinone El-Najjar, N., et al. J. Chem. Biol. 4, 97-107, (2011)

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In vitro Growth and Characterization of Stem Cells from Human Dental Pulp of Deciduous Versus Permanent Teeth. Eslaminejad MB J. Dent. (Tehran.) 7(4), 185-95, (2010)

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Role of protein kinase C and epidermal growth factor receptor signalling in growth stimulation by neurotensin in colon carcinoma cells. Müller, KM BMC Cancer 11, 421, (2011)

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Detection of human cytomegalovirus in motile spermatozoa and spermatogenic cells in testis organotypic culture. Naumenko VA Herpesviridae 2(1), 7, (2011)

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Characterization of the expression of cytokeratins 5, 8, and 14 in mouse thymic epithelial cells during thymus regeneration following acute thymic involution. Lee EN Anat. Cell Biol. 44(1), 14-24, (2011)

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Prognostic significance of heat shock protein 70 (HSP70) in patients with oral cancer. Tavassol F Head Neck Oncol. 3, 10, (2011)

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Influence of horse stable environment on human airways. Elfman L J. Occup. Med. Toxicol. 4, 10, (2009)

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Fibrin-based tissue engineering scaffolds enhance neural fiber sprouting and delay the accumulation of reactive astrocytes at the lesion in a subacute model of spinal cord injury. Johnson PJ J. Biomed. Mater. Res. A 92(1), 152-63, (2010)

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Long-term homeostasis of extracellular glutamate in the rat cerebral cortex across sleep and waking states. Dash MB J. Neurosci. 29(3), 620-9, (2009)

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Soluble factors from neocortical astrocytes enhance neuronal differentiation of neural progenitor cells from adult rat hippocampus on micropatterned polymer substrates. Oh J J. Biomed. Mater. Res. A 91(2), 575-85, (2009)

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Lung responses to secondary endotoxin challenge in rats exposed to pig barn air. Charavaryamath C J. Occup. Med. Toxicol. 3, 24, (2008)

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Suppression of astrovirus replication by an ERK1/2 inhibitor. Moser LA J. Virol. 82(15), 7475-82, (2008)

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Structural basis for c-di-GMP-mediated inside-out signaling controlling periplasmic proteolysis. Navarro MV PLoS Biol. 9(2), e1000588, (2011)

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Downregulation of Kv4.2 channels mediated by NR2B-containing NMDA receptors in cultured hippocampal neurons. Zhigang Lei et al Neuroscience 165, 350-62, (2010)

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Phosphorylation-independent regulation of the diguanylate cyclase WspR. Nabanita De et al PLoS Biol. 6, e67, (2008)

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Enhanced in vitro chondrogenesis of primary mesenchymal stem cells by combined gene transfer. Steinert AF Tissue Eng. Part A 15(5), 1127-39, (2009)

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A point mutation produced a class 3 aldehyde dehydrogenase with increased protective ability against the killing effect of cyclophosphamide. Ho KK Biochem. Pharmacol. 76(5), 690-6, (2008)

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Butein suppresses constitutive and inducible signal transducer and activator of transcription (STAT) 3 activation and STAT3-regulated gene products through the induction of a protein tyrosine phosphatase SHP-1. Pandey MK, Sung B, Ahn KS, et al. Mol. Pharmacol. 75(3), 525-33, (2009)

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Autocrine activation of neuronal NMDA receptors by aspartate mediates dopamine- and cAMP-induced CREB-dependent gene transcription. Luis E. F. Almeida et al J. Neurosci. 29, 12702-10, (2009)

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Combination therapy in treatment of experimental pulmonary aspergillosis: in vitro and in vivo correlations of the concentration- and dose- dependent interactions between anidulafungin and voriconazole by Bliss independence drug interaction analysis. Vidmantas Petraitis et al Antimicrob. Agents Chemother. 53, 2382-91, (2009)

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A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning. Florence Teulé et al Nat. Protoc. 4, 341-55, (2009)

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Axonal regeneration and neuronal function are preserved in motor neurons lacking ß-actin in vivo. Cheever TR PLoS ONE 6(3), e17768, (2011)

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The dynactin complex enhances the speed of microtubule-dependent motions of adenovirus both towards and away from the nucleus. Martin F. Engelke et al Viruses 3, 233-53, (2011)

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Super-resolution dynamic imaging of dendritic spines using a low-affinity photoconvertible actin probe. Izeddin, I PLoS ONE 6(1), e15611, (2011)

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Analysis of human immunodeficiency virus type 1 matrix binding to membranes and nucleic acids. Alfadhli A, Still A, and Barklis E J. Virol. 83(23), 12196-203, (2009)

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VEGF and RANKL regulation of NFATc1 in heart valve development. Michelle D. Combs et al Circ. Res. 105, 565-74, (2009)

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S100 calgranulin proteins S100A8, S100A9 and S100A12 are expressed in the inflamed gastric mucosa of Helicobacter pylori-infected children. Leach ST Can. J. Gastroenterol. 22(5), 461-4, (2008)

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Fine antigenic specificity and cooperative bactericidal activity of monoclonal antibodies directed at the meningococcal vaccine candidate factor h-binding protein. Beernink PT Infect. Immun. 76(9), 4232-40, (2008)

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Pulse-oximetry accurately predicts lung pathology and the immune response during influenza infection. Verhoeven D Virology 390(2), 151-6, (2009)

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Quantitative PCR for genetic markers of human fecal pollution. Shanks OC Appl. Environ. Microbiol. 75(17), 5507-13, (2009)

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The pseudomonas toxin pyocyanin inhibits the dual oxidase-based antimicrobial system as it imposes oxidative stress on airway epithelial cells Rada B, et al. J. Immunol. 181, 4883-4893, (2008)

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Lentiviral-mediated genetic correction of hematopoietic and mesenchymal progenitor cells from Fanconi anemia patients. Jacome A Mol. Ther. 17(6), 1083-92, (2009)

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Role of the Plasmodium export element in trafficking parasite proteins to the infected erythrocyte. Boddey JA Traffic 10(3), 285-99, (2009)

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Molecular cloning and characterization of a novel pyrethroid-hydrolyzing esterase originating from the Metagenome. Li G Microb. Cell Fact. 7, 38, (2008)

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In vivo stable tumor-specific painting in various colors using dehalogenase-based protein-tag fluorescent ligands. Kosaka N Bioconjug. Chem. 20(7), 1367-74, (2009)

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Ionic components of electric current at rat corneal wounds. Vieira AC PLoS ONE 6(2), e17411, (2011)

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Protective effects of mildronate in an experimental model of type 2 diabetes in Goto-Kakizaki rats. Edgars Liepinsh et al Br. J. Pharmacol. 157, 1549-56, (2009)

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Novel macromolecular inhibitors of human immunodeficiency virus-1 protease. Miklóssy, G Protein Eng. Des. Sel. 21(7), 453-61, (2008)

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Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis. Marlena Zielinska et al Int. J. Nanomedicine 6, 3163-72, (2011)

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The role of sialyl glycan recognition in host tissue tropism of the avian parasite Eimeria tenella. Lai L PLoS Pathog. 7(10), e1002296, (2011)

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Striking denervation of neuromuscular junctions without lumbar motoneuron loss in geriatric mouse muscle. Chai RJ PLoS ONE 6(12), e28090, (2011)

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Spontaneous development of a pancreatic exocrine disease in CD28-deficient NOD mice. Meagher C J. Immunol. 180(12), 7793-803, (2008)

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IFN-gamma reverses IL-2- and IL-4-mediated T-cell steroid resistance. Goleva E Am. J. Respir. Cell. Mol. Biol. 40(2), 223-30, (2009)

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Interferon-λ1 (interleukin-29) preferentially down-regulates interleukin-13 over other T helper type 2 cytokine responses in vitro. Shekar Srinivas et al Immunology 125, 492-502, (2008)

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Selenium nanoparticles inhibit Staphylococcus aureus growth. Tran PA Int. J. Nanomedicine 6, 1553-8, (2011)

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Detection of dynamic spatiotemporal response to periodic chemical stimulation in a Xenopus embryonic tissue. Kim Y, Joshi SD, Messner WC, et al. PLoS ONE 6(1), e14624, (2011)

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Role of glycocalyx in flow-induced production of nitric oxide and reactive oxygen species. Kumagai R Free Radic. Biol. Med. 47(5), 600-7, (2009)

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Site directed biotinylation of filamentous phage structural proteins. Smelyanski L Virol. J. 8, 495, (2011)

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L-type Ca2+ channel function is linked to dystrophin expression in mammalian muscle. Friedrich O PLoS ONE 3(3), e1762, (2008)

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Addressing reported pro-apoptotic functions of NF-kappaB: targeted inhibition of canonical NF-kappaB enhances the apoptotic effects of doxorubicin. Bednarski BK PLoS ONE 4(9), e6992, (2009)

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Inhibition of carboxylesterase activity of THP1 monocytes/macrophages and recombinant human carboxylesterase 1 by oxysterols and fatty acids. Crow, JA Biochim. Biophys. Acta 1801(1), 31-41, (2010)

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Analysis of apoptosis by cytometry using TUNEL assay. Darzynkiewicz Z, Galkowski D, and Zhao H Methods 44(3), 250-4, (2008)

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Use of long-term cultured embryoid bodies may enhance cardiomyocyte differentiation by BMP2. Kim YY Yonsei Med. J. 49(5), 819-27, (2008)

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Development of the Gateway system for cloning and expressing genes in Entamoeba histolytica. Abhyankar MM Parasitol. Int. 58(1), 95-7, (2009)

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RGS9 knockout causes a short delay in light responses of ON-bipolar cells. Herrmann R, Lee B, and Arshavsky VY PLoS ONE 6(11), e27573, (2011)

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Progressive tumor features accompany epithelial-mesenchymal transition induced in mitochondrial DNA-depleted cells. Naito A Cancer Sci. 99(8), 1584-8, (2008)

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Characterization of primary neurospheres generated from mouse ventral rostral hindbrain. Osterberg N Cell Tissue Res. 336(1), 11-20, (2009)

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Red Cabbage (Brassica oleracea) Ameliorates Diabetic Nephropathy in Rats. Hazem A. H. Kataya et al Evid. Based. Complement. Alternat. Med. 5, 281-7, (2008)

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Design and analysis of rhesus cytomegalovirus IL-10 mutants as a model for novel vaccines against human cytomegalovirus. Naomi J. Logsdon et al PLoS ONE 6, e28127, (2011)

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Saccharide/protein conjugate vaccines for Bordetella species: preparation of saccharide, development of new conjugation procedures, and physico-chemical and immunological characterization of the conjugates. Kubler-Kielb J, Vinogradov E, Ben-Menachem G, et al. Vaccine 26(29-30), 3587-93, (2008)

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Hippocampal interneurons co-express transcripts encoding the alpha7 nicotinic receptor subunit and the cannabinoid receptor 1. Morales, M Neuroscience 152(1), 70-81, (2008)

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ZEB1 limits adenoviral infectability by transcriptionally repressing the coxsackie virus and adenovirus receptor. Lacher MD Mol. Cancer 10, 91, (2011)

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Adiponectin-coated nanoparticles for enhanced imaging of atherosclerotic plaques. Almer G Int. J. Nanomedicine 6, 1279-90, (2011)

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Genetic diversity of heat-labile toxin expressed by enterotoxigenic Escherichia coli strains isolated from humans. Lasaro MA J. Bacteriol. 190(7), 2400-10, (2008)

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Attenuation of acridine mutagen ICR-191--DNA interactions and DNA damage by the mutagen interceptor chlorophyllin. Pietrzak M Biophys. Chem. 135(1-3), 69-75, (2008)

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Evidence of fibrinogen as a target of citrullination in IgM rheumatoid factor-positive polyarticular juvenile idiopathic arthritis. Gilliam BE Pediatr. Rheumatol. Online J. 9, 8, (2011)

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Aberrant anaplastic lymphoma kinase activity induces a p53 and Rb-dependent senescence-like arrest in the absence of detectable p53 stabilization. McDuff FK PLoS ONE 6(3), e17854, (2011)

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Bacteriophages with potential for inactivation of fish pathogenic bacteria: survival, host specificity and effect on bacterial community structure. Pereira C Mar. Drugs 9(11), 2236-55, (2011)

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Mouse early oocytes are transiently polar: three-dimensional and ultrastructural analysis. Kloc M Exp. Cell Res. 314(17), 3245-54, (2008)

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Peroxisome proliferator-activated receptor-gamma agonists suppress tissue factor overexpression in rat balloon injury model with paclitaxel infusion. Park JB PLoS ONE 6(11), e28327, (2011)

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Small compound 6-O-angeloylplenolin induces mitotic arrest and exhibits therapeutic potentials in multiple myeloma. Ying Liu et al PLoS ONE 6, e21930, (2011)

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Pseudomonas aeruginosa exploits lipid A and muropeptides modification as a strategy to lower innate immunity during cystic fibrosis lung infection. Cigana C PLoS ONE 4(12), e8439, (2009)

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Transport activity of the sodium bicarbonate cotransporter NBCe1 is enhanced by different isoforms of carbonic anhydrase. Schueler C PLoS ONE 6(11), e27167, (2011)

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Zinc oxide nanoparticles as selective killers of proliferating cells. Taccola L Int. J. Nanomedicine 6, 1129-40, (2011)

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Expression of RHOGTPase regulators in human myometrium. O'Brien M Reprod. Biol. Endocrinol. 6, 1, (2008)

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A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards. Sivaganesan M BMC Bioinformatics 9, 120, (2008)

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Fused eco29kIR- and M genes coding for a fully functional hybrid polypeptide as a model of molecular evolution of restriction-modification systems. Mokrishcheva, ML BMC Evol. Biol. 11, 35, (2011)

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Orthotopic transplantation of immortalized mesencephalic progenitors (CSM14.1 cells) into the substantia nigra of hemiparkinsonian rats induces neuronal differentiation and motoric improvement. Haas SJ J. Anat. 212(1), 19-30, (2008)

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CCL28 Induces Mucosal Homing of HIV-1-Specific IgA-Secreting Plasma Cells in Mice Immunized with HIV-1 Virus-Like Particles. Veronica Rainone et al PLoS ONE 6, e26979, (2011)

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Optimizing human synovial fluid preparation for two-dimensional gel electrophoresis. Chen CP Proteome Sci. 9, 65, (2011)

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The transcriptome of the nosocomial pathogen Enterococcus faecalis V583 reveals adaptive responses to growth in blood. Vebø HC PLoS ONE 4(11), e7660, (2009)

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Glycosylation of mucins present in gastric juice: the effect of helicobacter pylori eradication treatment. Radziejewska I Clin. Exp. Med. 11(2), 81-8, (2011)

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Phosphatase inhibitors with anti-angiogenic effect in vitro. Sylvest L APMIS 118(1), 49-59, (2010)

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Insoluble, speckled cytosolic distribution of retinoic acid receptor alpha protein as a marker of hepatic stellate cell activation in vitro. Mezaki Y J. Histochem. Cytochem. 57(7), 687-99, (2009)

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CD24 regulated gene expression and distribution of tight junction proteins is associated with altered barrier function in oral epithelial monolayers. Ye P BMC Cell Biol. 10, 2, (2009)

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Induction of cell-cell fusion by ectromelia virus is not inhibited by its fusion inhibitory complex. Erez N Virol. J. 6, 151, (2009)

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Astrocyte-derived tissue transglutaminase interacts with fibronectin: a role in astrocyte adhesion and migration? van Strien ME PLoS ONE 6(9), e25037, (2011)

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Cell cycle control and DNA damage response of conditionally immortalized urothelial cells. Dixon BP PLoS ONE 6(1), e16595, (2011)

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Separate basolateral and apical phosphatidylcholine secretion routes in intestinally differentiated tumor cells. Gotthardt, D World J. Gastroenterol. 15(46), 5821-6, (2009)

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Oxidative stress impairs the heat stress response and delays unfolded protein recovery. Adachi M PLoS ONE 4(11), e7719, (2009)

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