EMAIL THIS PAGE TO A FRIEND
A8531 Sigma

Bovine Serum Albumin

pH <5.0, powder

Synonym: Albumin bovine serum, BSA, Bovine albumin

Purchase

Properties

Related Categories Albumin, Albumins by Application, Biochemicals and Reagents, Bovine Serum Albumins, Ethanol Fractionated,
form   powder
mol wt   mol wt ~66 kDa
  mol wt ~66,000
packaging   vial of 50 mg
impurities   ~0.3% dithiothreitol
pH   <5.0
suitability   suitable for (Suitable for use as a gel filtration marker)
storage temp.   −20°C

Description

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Application

Albumin from bovine serum was used:
• for blocking of nonspecific immunoglobulin during immunohistochemical reactions.
• to coat microplates for blocking non-specific binding during I-ELISA (Indirect Enzyme-linked Immunosorbent Assay)
• as molecular mass standard in analytical gel filtration chromatography for Superdex® 75 10/300 GL gel filtration column.
• For measuring fluorescence for PR1 (phytofluor red 1) by using Fluorescence correlation spectroscopy (FCS)
• As a reference mixture along with horse Mb in Size Exclusion Chromatography
It can be used as a gel filtration molecular weight marker. It can be used in medical studies to quantify binding capacity and adsorption rate of protein enrichment and separation medium critical in proteomic research.

Gel filtration molecular weight marker

Biochem/physiol Actions

Bovine serum albumin also refers as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated and non-treated microwells. It can also triggers the insulin dependent diabetes mellitus.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Legal Information

Superdex is a registered trademark of GE Healthcare

Protocols & Applications

Select the right albumin for your application with the Albumin Selection Guide

Price and Availability


BioAnalysis

Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?