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A8580 Sigma

Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit

IgG fraction of antiserum, PBS suspension

Synonym: Anti-Glutathione-S-Transferase, Anti-GST

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Properties

Related Categories Affinity Chromatography, Alphabetical Index, Antibodies, Antibodies to Peptide and Protein Fusion Tags, GD-GL,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   PBS suspension
mol wt   antigen mol wt 27.5  kDa
does not react with   rabbit, human, porcine, rat, bovine
application(s)   ELISA: suitable
  immunoprecipitation: suitable
conjugate   agarose conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Immunogen

recombinant GST from Schistosoma japonicum expressed in E. coli.

Preparation Note

Prepared by coupling cyanogen bromide-activated agarose at 7-8 mg antibody per mL resin volume.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Application

Immunoprecipitation was performed on NIH 3T3 cells transiently transfected with GST fusion proteins. IP was performed using agarose-linked rabbit anti-GST IgG with mixing for 2 hours at 4 degrees.

Rabbit Anti-Glutathione-S-Transferase (GST)-Agarose antibody can be used for ELISA, immunoaffinity purification and immunoprecipitation assays.

General description

Recombinant target proteins are often expressed as a fusion product with Glutathione-S-Transferase (GST) tags using various expression vector constructs. Thus, antibodies directed against the GST tags of the recombinant constructs can facilitate the purification and study of target proteins.
Rabbit Anti-Glutathione-S-Transferase (GST)-Agarose antibody does not recognize GST from rabbit liver, porcine liver, bovine liver, rat liver or human placenta when tested by ELISA.

The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.

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Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
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