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A8599 Sigma

Monoclonal Anti-Apolipoprotein E antibody produced in mouse

~1 mg/mL, clone E6D7, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-Apo E

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Properties

Related Categories AO-AQ, Alphabetical Index, Antibodies, Antibodies for Alzheimer′s Disease, Antibodies for Neurobiology,
species reactivity   human
application(s)   immunohistochemistry: 1:100-1:10,000 using fixed tissues pretreated with 70% formic acid for 10-30 minutes at room temperature.
  immunoprecipitation: suitable
  indirect ELISA: suitable
  western blot: 1:102-1:103
clone   E6D7, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... APOE(348)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide spanning the polymorphic amino acid position 158 of ApoE.

General description

Mutations in the APP gene in conjunction with the influence of ApoE appears to influence amyloid metabolism. Reacts to the E2, E3, and E4 isoforms of human apoliporotein E.

There are three major isoforms of ApoE: E2, E3 and E4. Studies have reported that ApoE is involved in development of Alzheimer′s Disease (AD). The E4 isoform, in particular, is elevated in both late onset and sporadic AD patients.

Antigen Background

APOE binds to lipids forming lipoproteins called VLDL in the lever where it functions in the transport of triglycerides.

Physical form

Solution in phosphate buffered saline.

Application

Mouse monoclonal anti-apolipoprotein E antibody has been used for immunohistochemical assays at 1:1,000. The antibody can also be used for western blot (1:100-1:1,000), immunoprecipitation and indirect ELISA.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
Protocols & Articles

Articles

Alzheimer's Disease

Alzheimer's disease (AD) is the most common cause of dementia in the elderly and is characterized by gradual loss of cognitive functions. Hallmark pathohistological findings of AD include widespread ...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 4–8
Keywords: Alzheimer Disease, Gene expression, Genetic, Genetics, Inflammation, Metabolism, Phosphorylations

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Peer-Reviewed Papers
15

References

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