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A8656 Sigma

Alcohol Dehydrogenase from Saccharomyces cerevisiae

Synonym: ADH, Alcohol Dehydrogenase from yeast, Alcohol:NAD+ oxidoreductase



Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates More...
mol wt   mol wt ~150,000
packaging   vial of 25 mg
storage temp.   −20°C



Alcohol Dehydrogenase from Saccharomyces cerevisiae is used for gel filtration chromatography and as a gel filtration molecular weight marker. It has been used in bioelectrochemical research to investigate the use of diamond nanoparticles as a surface for protein loading.

Biochem/physiol Actions

ADH (alcohol dehydrogenase) is one of the first enzymes to be isolated and purified. NAD+ is its coenzyme. Three isozymes of yeast ADH, that is, yeast alcohol dehydrogenase-1, 2 and 3 (YADH-1, -2, -3) have been identified. YADH-1 is expressed during anaerobic fermentation, YADH-2 is expressed in the cytoplasm and YADH-3 is localized to the mitochondria. A 141kDa tetramer containing 4 equal subunits. The active site of each subunit contains a zinc atom. Each active site also contains 2 reactive sulfhydryl groups and a histidine residue.

Isoelectric point: 5.4-5.8

Optimal pH: 8.6-9.0

Substrates: Yeast ADH is most active with ethanol and its activity decreases as the size of the alcohol increases or decreases. Branched chain alcohols and secondary alcohols also have very low activity.

KM (ethanol) = 2.1 × 10-2 M
KM (methanol = 1.3 × 10-1 M
KM (isopropanol) = 1.4 × 10-1 M

Inhibitors: Compounds that react with free sulfhydryls, including N-alkylmaleimides and iodoacetamide.
Zinc chelator inhibitors, including 1,10-phenanthroline,
8-hydroxyquinoline, 2,2′-dipyridyl, and thiourea.
Substrate analogue inhibitors, including β-NAD analogs, purine and pyrimidine derivatives, chloroethanol, and fluoroethanol.

Extinction Coefficient: E1% = 14.6 (water, 280 nm)

Price and Availability

Available in ELITE Grade

Tools for Large Molecule BioAnalysis

Amicon Pro Purification System
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da

Gel filtration chromatography is an established method for determining the size and molecular mass of proteins. Fractionation is based on the diffusion of molecules into the pores of the resin. Large...
Keywords: Chromatography, Diffusion, Filtration, Fractionation, High performance liquid chromatography, Size-exclusion chromatography


Enzymatic Assay of Alcohol Dehydrogenase (EC

This procedure may be used for Alcohol Dehydrogenase products, except for Alcohol Dehydrogenase, Insoluble Enzyme attached to beaded agarose (Catalog No. A2529).
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

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