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A8667 Sigma

Anti-Human IgG (whole molecule)−Peroxidase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Human IgG Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies,
biological source   goat
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
species reactivity   human
application(s)   direct ELISA: 1:30,000
  dot blot: 1:80,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

human IgG

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal

Application

Goat Anti-Human IgG (whole molecule)-Peroxidase antibody has been used for ELISA. The product can also be used for dot blot (1:80,000) and immunohistochemistry (1:200).

Specificities of anti-human HIV-1 envelope antibodies was determined by ELISA using HRP-conjugated goat anti-human IgG at a dilution of 1:1000.

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Goat Anti-Human IgG (whole molecule)-Peroxidase antibody binds to human IgG.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
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Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
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Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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