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A8786 Sigma

Anti-Mouse IgM (μ-chain specific)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:10,000
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Purified mouse IgM

Packaging

1 mL in glass bottle

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 0.01% thimerosal.

Application

Anti-Mouse IgM (μ-chain specific)-Peroxidase antibody is suitable for use in western blot (1:5000) and ELISA (1:2000) .

ELISA was performed to identify auto-antibodies that bind to glomerular antigen extracts from mouse kidneys using HRP-conjugated goat anti-mouse IgM mu chain specific antibody at a dilution of 1:4000.

General description

IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory . This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice . Anti-Mouse IgM (μ-chain specific)-Peroxidase antibody is specific for mouse IgM when tested against purified mouse IgA, IgM, and IgG.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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