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A8794 Sigma

Anti-Human Polyvalent Immunoglobulins (whole molecule)–Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Human Polyvalent Ig Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:30,000 using IgG
  dot blot: 1:20,000 using direct chemiluminescence assay using 20 ng human IgG, IgA, or IgM/dot. Luminol plus enhancer was used a substrate.
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Human immunoglobulins

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.01% thimerosal

Application

Anti-Human Polyvalent Immunoglobulins (whole molecule)-Peroxidase antibody is suitable for use in dot blot (1:20,000, by direct chemiluminescence assay using 20 ng human IgG, IgA, or IgM/dot and Luminol plus enhancer as substrate). The antibody may also be used in direct ELISA (1:30,000 using IgG), immunohistochemistry (1:200, using formalin-fixed, paraffin-embedded sections), immunoelectrophoresis and Ouchterlony double diffusion assays.

ELISA was performed using HRP-conjugated rabbit anti-human polyvalent IgG to identify human intravenous polyclonal IgGs that bind specifically to native LukS-PV and LukF-PV proteins.

General description

Human immunoglobulins are glycoproteins that regulate immune responses to infections and allergies. Anti-human polyvalent immunoglobulins can be used for serological analyses in diseased population groups . These antibodies may also be used for studying xenotransplantation models and immunological disorders such as rheumatoid arthritis . Anti-Human Polyvalent Immunoglobulins (whole molecule)-Peroxidase antibody is specific for human antibodies, and reacts with purified human IgA, IgG, IgM, Bence Jones κ, and Bence Jones lambda myeloma proteins.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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