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A8794 Sigma

Anti-Human Polyvalent Immunoglobulins (whole molecule)–Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Human Polyvalent Ig Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:30,000 using IgG
  dot blot: 1:20,000 using direct chemiluminescence assay using 20 ng human IgG, IgA, or IgM/dot. Luminol plus enhancer was used a substrate.
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Human immunoglobulins

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.01% thimerosal

Application

Anti-Human Polyvalent Immunoglobulins (whole molecule)-Peroxidase antibody is suitable for use in dot blot (1:20,000, by direct chemiluminescence assay using 20 ng human IgG, IgA, or IgM/dot and Luminol plus enhancer as substrate). The antibody may also be used in direct ELISA (1:30,000 using IgG), immunohistochemistry (1:200, using formalin-fixed, paraffin-embedded sections), immunoelectrophoresis and Ouchterlony double diffusion assays.

ELISA was performed using HRP-conjugated rabbit anti-human polyvalent IgG to identify human intravenous polyclonal IgGs that bind specifically to native LukS-PV and LukF-PV proteins.

General description

Human immunoglobulins are glycoproteins that regulate immune responses to infections and allergies. Anti-human polyvalent immunoglobulins can be used for serological analyses in diseased population groups . These antibodies may also be used for studying xenotransplantation models and immunological disorders such as rheumatoid arthritis . Anti-Human Polyvalent Immunoglobulins (whole molecule)-Peroxidase antibody is specific for human antibodies, and reacts with purified human IgA, IgG, IgM, Bence Jones κ, and Bence Jones lambda myeloma proteins.

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RIDADR 
NONH for all modes of transport
WGK Germany 
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Articles

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