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A8811 Sigma

Aldolase from rabbit muscle

ammonium sulfate suspension, 10-20 units/mg protein

Synonym: D-Fructose-1,6-bisphosphate-D-glyceraldehyde-3-phosphate-lyase, Fructose-diphosphate Aldolase

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Properties

Related Categories 4.1.x.x C-C bonds, 4.x.x.x Lyases, Application Index, Biochemicals and Reagents, Cell Biology,
form   ammonium sulfate suspension
foreign activity   glyceraldehyde-3-phosphate dehydrogenase ≤0.03%
  lactic dehydrogenase ≤0.03%
  phosphoglucose isomerase ≤0.6%
  pyruvate kinase ≤0.1%
  triosephosphate isomerase ≤0.05%
storage temp.   2-8°C

Description

Analysis Note

Protein determined by biuret.

Packaging

100, 200, 1000 units in poly bottle

5000 units in glass bottle

Unit Definition

One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C.

Physical form

Crystalline suspension in 2.5 M (NH4)2SO4, 0.01 M Tris, pH 7.5, 0.001 M EDTA

Application

Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 .

Biochem/physiol Actions

Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β 8 barrel fold .

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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