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  • A8914 - Anti-Rabbit IgG (whole molecule)−Agarose antibody produced in goat

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A8914 Sigma

Anti-Rabbit IgG (whole molecule)−Agarose antibody produced in goat

affinity isolated antibody, saline suspension

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Properties

Related Categories Agarose, Alphabetical Index, Antibodies, Antibody Purification and Characterization, Antibody-Agaroses,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   saline suspension
extent of labeling   ~1 mg per mL
application(s)   immunoelectrophoresis: suitable
matrix   Cross-linked 4% beaded agarose
matrix activation   cyanogen bromide
matrix spacer   1 atom
capacity   ≥0.25 mg/mL, resin binding capacity (rabbit IgG)
conjugate   agarose conjugate
storage temp.   2-8°C

Description

General description

IgG antibodies are found in all body fluids and are capable of fighting bacterial and viral infection. It is the only type of antibody capable of crossing the placenta and immunising the foetus. Anti-rabbit IgG (whole molecule)-agarose antibody can be used in immunoprecipitation. IgGs are added to nuclear extracts of breast cancer cell lines then immunoprecipitation carried out. Anti-rabbit IgG (whole molecule)-agarose antibody can react specifically to all rabbit immunoglobulin.

Immunogen

Purified rabbit IgG.

Physical form

Suspension in 0.5 M NaCl containing 0.01% thimerosal.

Application

Protein depletion from mouse intestinal cytosol samples was accomplished by incubating the samples with a specific primary and the pulling down with agarose-linked goat anti-rabbit IgG.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer - Search 80,000 Antibodies at Sigma-Aldrich

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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