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A8919 Sigma

Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, GM-GO, Goat Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:30,000
  dot blot: 1:2,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
  western blot (chemiluminescent): 1:160,000
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Purified goat IgG

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.01% thimerosal.

Application

Anti-goat IgG (whole molecule)-peroxidase antibody (diluted 1: 1000) can be used as a secondary antibody in immunoblotting. It can also be used in ELISA and dot blot.

Transfected NIH3T3 cell lysates were subjected to western blot where blots were probed with HRP-conjugated rabbit anti-goat IgG as the secondary antibody.

General description

IgG antibodies are the smallest antibody isotype found in blood and extracellular fluid, capable of fighting bacterial and viral infection. It also regulates several functions like placental transport, cell surface-receptor binding and complement activation.Anti-goat IgG (whole molecule)-peroxidase antibody (diluted 1: 1000 in 5% in TBS-pH 8) can be used as a secondary antibody in western blotting. It can also be used in immunohistology. Rabbit anti-goat IgG (whole molecule)-peroxidase antibody reacts specifically with goat IgG.

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