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A8936 Sigma

Alanine Racemase from Bacillus stearothermophilus

lyophilized powder, ≥10 IU/mg solid



Related Categories 5.1.x.x Racemases and epimerases, 5.x.x.x Isomerases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates More...
form   lyophilized powder
mol wt   Mr 78 kDa (2 subunits 39 kDa each)
storage temp.   −20°C


Unit Definition

One unit will convert 1.0 μmole of D-alanine to L-alanine per minute at pH 10.5 at 30 °C in a coupled assay system with L-alanine dehydrogenase.

Physical form

Lyophilized from 50 mM phosphate buffer, pH 7.5


Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-14C]alanine to a racemic mixture of L/D- [14C]alanine .

Biochem/physiol Actions

Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase. Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.

Price and Availability

Biomedical Applications
Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Enzymatic Assay of D-Alanine Racemase (EC

To standardize a procedure for the enzymatic assay of D-Alanine Racemase, Sigma Product Number A8936, at Sigma-Aldrich St. Louis.
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers


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