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A8957 Sigma

Adenosine-5′-triphosphate Sulfurylase from Saccharomyces cerevisiae

lyophilized powder, ≥1.0 units/mg protein

Synonym: ATP:Sulfate adenylyltransferase

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Properties

Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   lyophilized powder
composition   Protein, ≥40% modified Warburg-Christian
foreign activity   hexokinase ≤5%
storage temp.   −20°C

Description

Unit Definition

One unit will produce 1.0 μmole of ATP from APS and inorganic pyrophosphate per min at pH 8.0 at 30°C.

Physical form

Contains citrate buffer salts

Application

Adenosine-5′-triphosphate sulfurylase (ATP sulfurylase) may be used to study sulfur metabolism and hydrogen sulfide biosynthesis. ATP sulfurylase is also used in pyrosequencing. The enzyme has been used along with luciferase in a bioluminescent assay for inorganic sulfate quantitation in freshwater. Inorganic sulfate is converted, by ATP sulfurylase, into adenosine-5′-phosphosulfate and inorganic pyrophosphate, with consumption of ATP. The remaining ATP is used as a co-factor in the reaction catalyzed by firefly luciferase, generating photons of visible light as a co-product. The light output is inversely proportional to the content of inorganic sulfate.

Biochem/physiol Actions

Adenosine-5′-triphosphate sulfurylase catalyzes the first intracellular reaction of sulfate assimilation, which forms adenosine-5′-phosphosulfate (APS) from inorganic sulfate and ATP. It is involved in sulfate activation and the biosynthesis pathway of sulfur-containing amino acids. The expression of ATP sulfurylase is dependent on the formation of the MET4-MET28-MET31 and MET4-MET28-MET32 complexes on its 5′ upstream region .

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All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

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