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  • A8967 - Anti-Amyloid Precursor Protein, N-Terminal antibody produced in rabbit

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A8967 Sigma

Anti-Amyloid Precursor Protein, N-Terminal antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-APP

Properties

Related Categories AM-AN, Alphabetical Index, Antibodies, Antibodies for Alzheimer′s Disease, Antibodies for Neurobiology,
species reactivity   rat, mouse, human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formic acid-treated sections of human Alzheimer′s disease (AD) brain
  microarray: suitable
  western blot: 1:1,000 using rat brain extract or supernatant of 293T cells secreting APP.
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 95-100 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... APP(351)
mouse ... App(11820)
rat ... App(54226)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the N-terminal of human APP695 (amino acids 46-60) conjugated to KLH.

General description

Amyloid precursor proteins (APPs) are transmembrane glycoproteins that are found in a wide range of tissues. APPs have 3 main isoforms, namely, APP695, APP751 and APP770 that are derived from alternative splicing events in cells. Accumulation of the cleavage products of APP, such as the β-amyloid peptide, can cause Alzheimer′s disease.

The immunogen sequence is identical to the APP isoforms APP751 and APP770 and is highly conserved (single amino acid substitution) in rat and mouse APP695. The antibody recognizes APP695, APP751 and APP770.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Rabbit Anti-Amyloid Precursor Protein, N-Terminal antibody can be used for immunostaining primary neuronal cultures. The antibody can also been used for western blot and microarray studies.

SH-SY5Y cell lysates were analyzed by western blot using rabbit anti-Amyloid Precursor Protein, C-Terminal as the primary antibody at a 1:500 dilution.

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