Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories	Alphabetical Index, Antibodies, Antibody Conjugates, FLAG Secondary Antibodies, FLAG System, MO-MR, Mouse Secondary Antibodies and Conjugates, Peroxidase Conjugates, Peroxidase Labeled Antibodies, Phosphorylation Analysis, Post-Translational Modification, Primary Antibodies, Protein Electrophoresis, Proteomics, Purification and Detection, Recombinant Protein Expression and Analysis, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies, Secondary Antibodies and Labeled Streptavidin, Western Blotting More...
antibody form	IgG fraction of antiserum
clone	polyclonal
form	buffered aqueous solution
application(s)	direct ELISA: 1:40,000 using using 5 μg/ml of mouse IgG for coating and OPD substrate
	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
	western blot (chemiluminescent): 1:80,000-160,000
shipped in	dry ice
storage temp.	−20°C

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Description

Immunogen

Purified mouse IgG

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Specificity

Binds all mouse Igs

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal.

Application

Anti-Mouse IgG (whole molecule)–Peroxidase antibody may be used in direct ELISA at a working antibody dilution of 1:40,000. For immunoblotting, a dilution of 1:80,000 to 1:160,000 may be used. A working antibody dilution of 1:200 is recommended for immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil or human appendix sections. Mouse IgG-peroxidase antibody was used at a dilution of 1:20 for immunohistochemistry of mice muscle tissue sections; a dilution of 1:500 was used for South-western blotting in the same study.¹ For immunoblotting of human skeletal muscle tissue extracts, a dilution of 1:5000 was used.²

Epidermis protein extracts were generated from human mammary surgery specimens and subjected to western blot using HRP-conjugated rabbit anti-mouse IgG as the secondary at a 1:80000 dilution in TBSt/5% milk.

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections³
Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for all mouse IgG subclasses. IgG is purified from mouse serum by fractionation and ion exchange chromatography. The purified IgG is then conjugated to peroxidase by protein cross-linking with 0.2% glutaraldehyde.