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A9045 Sigma

Agarose, low gelling temperature

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

Synonym: 2-Hydroxyethyl agarose



Related Categories Agarose, Cell Culture, Companion Products and Reagents, Core Bioreagents, Insect Platform,
product line   BioReagent
solubility   H2O: soluble10 mg/mL (with heat)
suitability   suitable for cell culture
  suitable for insect cell culture
  suitable for plant cell culture


Frequently Asked Questions

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Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

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Safety & Documentation

Safety Information

WGK Germany 

Protocols & Articles

Related Content

Agarose Selection Guide

From our library of Related Content, Sigma-Aldrich presents Agarose Selection Guide
Keywords: Cell culture, Electrophoresis, Immunodiffusion, Immunoelectrophoresis, Molecular biology, Protein electrophoresis, Separation

Peer-Reviewed Papers


Set your institution to view full text papers.

A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels. Wieslander, L. Anal. Biochem. 98, 305, (1979)


Agarose gel electrophoresis. Johansson B G. Scand. J. Clin. Lab. Invest. 29(S124), 7-19, (1972)

Agar and agarose biotechnological applications. Armisen R. Hydrobiologia 221(1), 157-66, (1991)

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Fire A, Xu S, Montgomery MK, et al. Nature 391(6669), 806-11, (1998)


A cytochemical staining procedure for succinate dehydrogenase activity in pre-ovulatory mouse oocytes embedded in low gelling temperature agarose. Boerjan ML, Baarends WM, and Ruven HJ J. Histochem. Cytochem. 39(1), 135-8, (1991)


Magnitude and direction of vesicle dynamics in growing pollen tubes using spatiotemporal image correlation spectroscopy and fluorescence recovery after photobleaching. Jérôme Bove et al Plant Physiol. 147, 1646-58, (2008)


Fluorescent microangiography is a novel and widely applicable technique for delineating the renal microvasculature. Advani A, Connelly KA, Yuen DA, et al. PLoS ONE 6(10), e24695, (2011)


Photothermal optical coherence tomography of epidermal growth factor receptor in live cells using immunotargeted gold nanospheres. Skala MC, Crow MJ, Wax A, et al. Nano Lett. 8(10), 3461-7, (2008)


Doxycycline-mediated inhibition of choroidal neovascularization. Samtani S Invest. Ophthalmol. Vis. Sci. 50(11), 5098-106, (2009)


Regression-based identification of behavior-encoding neurons during large-scale optical imaging of neural activity at cellular resolution. Miri A, Daie K, Burdine RD, et al. J. Neurophysiol. 105(2), 964-80, (2011)


ATP-dependent but proton gradient-independent polyphosphate-synthesizing activity in extraradical hyphae of an arbuscular mycorrhizal fungus. Tani C, Ohtomo R, Osaki M, et al. Appl. Environ. Microbiol. 75(22), 7044-50, (2009)


Y-27632 enhances differentiation of blastocyst like cystic human embryoid bodies to endocrinologically active trophoblast cells on a biomimetic platform. Sivasubramaiyan K, Totey S, Bhat V, et al. J. Biomed. Sci. 16, 88, (2009)


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