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A9045 Sigma

Agarose, low gelling temperature

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

Synonym: 2-Hydroxyethyl agarose

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Properties

Related Categories Agarose, Cell Culture, Companion Products and Reagents, Core Bioreagents, Insect Platform,
product line   BioReagent
solubility   H2O: soluble10 mg/mL (with heat)
suitability   suitable for cell culture
  suitable for insect cell culture
  suitable for plant cell culture

Description

Frequently Asked Questions

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Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

Agarose, a low gelling temperature derivative, is the suitable reagent for:
• plant cell culture studies
• insect cell culture studies
• cell culture studies

It may be used for the following studies:
• Recovery of defined RNA and DNA fractions after electrophoretic separation.1
• Cytochemical staining procedure to investigate the succinate dehydrogenase (SDH) activity in pre-ovulatory mouse oocytes.2
• Purification of RNA in Caenorhabditis elegans by electrophoresis.3

General description

Agarose is an algal polysaccharide. Agarose is a thermoreversible, ion-dependent gelling agent.4 Agarose gel electrophoresis is useful for the clinical routine analyses of proteins in plasma and other body fluids.5 It is a low gelling temperature derivative with unique gelling properties. Gels forms at <30°C, remelt at temperatures in excess of 60°C. Gels exhibit excellent clarity and are particularly useful for the preparation of media containing heat-labile materials.

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Safety & Documentation

Safety Information

WGK Germany 
3

Protocols & Articles

Related Content

Agarose Selection Guide

From our library of Related Content, Sigma-Aldrich presents Agarose Selection Guide
Keywords: Cell culture, Electrophoresis, Immunodiffusion, Immunoelectrophoresis, Molecular biology, Protein electrophoresis, Separation

Peer-Reviewed Papers

References

Set your institution to view full text papers.

1. A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels. Wieslander, L. Anal. Biochem. 98, 305, (1979)

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2. A cytochemical staining procedure for succinate dehydrogenase activity in pre-ovulatory mouse oocytes embedded in low gelling temperature agarose. Boerjan ML, Baarends WM, and Ruven HJ J. Histochem. Cytochem. 39(1), 135-8, (1991)

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3. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Fire A, Xu S, Montgomery MK, et al. Nature 391(6669), 806-11, (1998)

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4. Agar and agarose biotechnological applications. Armisen R. Hydrobiologia 221(1), 157-66, (1991)

5. Agarose gel electrophoresis. Johansson B G. Scand. J. Clin. Lab. Invest. 29(S124), 7-19, (1972)

Magnitude and direction of vesicle dynamics in growing pollen tubes using spatiotemporal image correlation spectroscopy and fluorescence recovery after photobleaching. Jérôme Bove et al Plant Physiol. 147, 1646-58, (2008)

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Fluorescent microangiography is a novel and widely applicable technique for delineating the renal microvasculature. Advani A, Connelly KA, Yuen DA, et al. PLoS ONE 6(10), e24695, (2011)

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Photothermal optical coherence tomography of epidermal growth factor receptor in live cells using immunotargeted gold nanospheres. Skala MC, Crow MJ, Wax A, et al. Nano Lett. 8(10), 3461-7, (2008)

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Doxycycline-mediated inhibition of choroidal neovascularization. Samtani S Invest. Ophthalmol. Vis. Sci. 50(11), 5098-106, (2009)

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Regression-based identification of behavior-encoding neurons during large-scale optical imaging of neural activity at cellular resolution. Miri A, Daie K, Burdine RD, et al. J. Neurophysiol. 105(2), 964-80, (2011)

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ATP-dependent but proton gradient-independent polyphosphate-synthesizing activity in extraradical hyphae of an arbuscular mycorrhizal fungus. Tani C, Ohtomo R, Osaki M, et al. Appl. Environ. Microbiol. 75(22), 7044-50, (2009)

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Y-27632 enhances differentiation of blastocyst like cystic human embryoid bodies to endocrinologically active trophoblast cells on a biomimetic platform. Sivasubramaiyan K, Totey S, Bhat V, et al. J. Biomed. Sci. 16, 88, (2009)

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