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  • A9046 - Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit

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A9046 Sigma

Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, CE-CH, Chicken Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies,
biological source   rabbit
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
species reactivity   chicken
application(s)   direct ELISA: 1:30,000
  dot blot: 1:160,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Immunogen

purified chicken IgG

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal

Application

Proteins isolated from rabbit glomeruli by iron oxide magnetization were subjected to western blot analysis using HRP-conjugated rabbit anti-chicken IgY as the secondary antibody.

Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody has been used for immunoprecipitation and western blot (1:2,000) assays. The antibody can also be used for direct ELISA (1:30,000), dot blot (1:12,000-1:15,000) and IHC (1:1,000) applications.

General description

Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications.
Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody binds to chicken IgG.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Will Product A9046, Anti-Chicken IgY, also recognize chicken IgG?
This antibody (and possibly other anti-chicken antibodies) recognizes both chicken IgG from serum and chicken IgY from egg yolk, because these immunoglobulins are structurally very similar.
How is chicken IgY different from chicken IgG?
Chicken IgG refers to antibodies from the chicken serum and chicken IgY antibodies are from the yolk.
Product A9046, Anti-Chicken IgY, is peroxidase conjugated.  Is this similar to horseradish peroxidase?
The peroxidase used to make this product is indeed peroxidase from horseradish (HRP).
Do we have additional anti-chicken IgY conjugates?
We provide anti-chicken IgY antibodies that are conjugated to alkaline phosphatase (Product No. A9171) and FITC (Product No. F8888).  We also provide the unconjugated anti-chicken IgY antibody (Product No. C2288).
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Protocols & Articles

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Peer-Reviewed Papers
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References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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