Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
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MDL number MFCD00162225
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Properties
| Related Categories | Alphabetical Index, Antibodies, CE-CH, Chicken Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies, |
| antibody form | affinity isolated antibody |
| clone | polyclonal |
| form | buffered aqueous solution |
| application(s) | direct ELISA: 1:30,000 |
| dot blot: 1:12,000-1:15,000 | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 | |
| western blot (chemiluminescent): 1:160,000 |
Description
Frequently Asked Questions
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Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Specificity
Binds chicken IgY (IgG).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal
Application
Proteins isolated from rabbit glomeruli by iron oxide magnetization were subjected to western blot analysis using HRP-conjugated rabbit anti-chicken IgY as the secondary antibody. 1
Price and Availability
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